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PROCESS PARAMETERS STUDY OF α-AMYLASE PRODUCTION IN A PACKED-BED BIOREACTOR UNDER SOLID-STATE FERMENTATION WITH POSSIBILITY OF TEMPERATURE MONITORING.
MedLine Citation:
PMID:  22509847     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Production of α-amylase in a laboratory-scale packed-bed bioreactor by Bacillus sp. KR-8104 under solid-state fermentation (SSF) with possibility of temperature control and monitoring was studied using wheat bran (WB) as a solid substrate. The simultaneous effects of aeration rate, initial substrate moisture, and incubation temperature on α-amylase production were evaluated using response surface methodology (RSM) based on a Box-Behnken design. The optimum conditions for attaining the maximum production of α-amylase were 37°C, 72% (w/w) initial substrate moisture, and 0.15 L/min aeration. The average enzyme activity obtained under the optimized conditions was 473.8 U/g dry fermented substrate. In addition, it was observed that the production of enzyme decreased from the bottom of the bioreactor to the top.
Authors:
Sorour Derakhti; Seyed Abbas Shojaosadati; Maryam Hashemi; Khosro Khajeh
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Preparative biochemistry & biotechnology     Volume:  42     ISSN:  1532-2297     ISO Abbreviation:  Prep. Biochem. Biotechnol.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9607037     Medline TA:  Prep Biochem Biotechnol     Country:  England    
Other Details:
Languages:  eng     Pagination:  203-16     Citation Subset:  IM    
Affiliation:
a Biotechnology Group, Chemical Engineering Department , Tarbiat Modares University , Tehran , Iran.
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