| Procedure for the embedment and ultrastructural visualization of cells cultured on plastic microtest plates. | |
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MedLine Citation:
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PMID: 321697 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The polystyrene Microtest plate has served as an excellent means of quantitation in the interaction of immunological, chemotherapeutic and radiobiological treatments with cultured cells. In order to assess the accompanying ultrastructural changes it was necessary to develop a technique which allowed cells grown in the wells of the plates to be embedded for electron microscopy. Conventional epoxy resin embedding techniques require the use of propylene oxide as a clearing agent. Unfortunately propylene oxide is a solvent of the polystyrene plates. By the substitution of absolute ethanol for propylene oxide, toluidine blue staining during the procedure and other preparative techniques it was feasible to prepare reacted cells grown in Microtest plates for electron microscopy. |
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Authors:
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T M Liszczak; J P Phillips; P L Kornblith |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of immunological methods Volume: 15 ISSN: 0022-1759 ISO Abbreviation: J. Immunol. Methods Publication Date: 1977 |
Date Detail:
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Created Date: 1977-05-25 Completed Date: 1977-05-25 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 131-4 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Immunologic Techniques* Methods Microscopy, Electron Polystyrenes* |
| Chemical | |
Reg. No./Substance:
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0/Polystyrenes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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