| Procaine inhibits the erythroid differentiation of MEL cells by blocking commitment: possible involvement of calcium metabolism. | |
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MedLine Citation:
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PMID: 6793604 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The action of procaine on the terminal erythroid differentiation of murine erythroleukemia (MEL) cells has been investigated at the level of individual cells. At concentrations (7 X 10-4 M) which had no inhibitory effect on cell growth, pretreatment of these cells with procaine for 12-24 hr caused a pronounced inhibition ( greater than 90%) of commitment of terminal erythroid differentiation of dimethyl sulfoxide (DMSO)-treated cells. Simultaneous treatment of MEL cells with DMSO and procaine, however, resulted to only slight inhibition (less than 20%) of commitment. Blockade of commitment by procaine pretreatment appears to be general since it was observed in cells treated with other inducers (6-thioguanine, dimethylformamide). Procaine pretreatment did not abolish the ability of MEL cells to complete the "latent period" and commit upon the removal of the block. Reversal of procaine inhibition of commitment was obtained by the addition of either CaCl2 (1.0 mM), calcium ionophore A23817 (1 microgram/ml), but not of MgCl2 (1.0 mM). From these data we conclude that procaine inhibits the terminal erythroid differentiation of MEL cells by blocking an event or process required for commitment which occurs prior to commitment itself. Our results suggest that this process involves calcium metabolism. |
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Authors:
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A S Tsiftsoglou; A A Mitrani; D E Housman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cellular physiology Volume: 108 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1981 Sep |
Date Detail:
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Created Date: 1981-12-21 Completed Date: 1981-12-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 327-35 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcimycin / pharmacology Calcium / metabolism* Cell Differentiation / drug effects* Cell Division / drug effects Cell Line Dimethyl Sulfoxide / antagonists & inhibitors Dose-Response Relationship, Drug Erythropoiesis / drug effects* Friend murine leukemia virus Leukemia, Erythroblastic, Acute / pathology Mice Procaine / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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CA 17575/CA/NCI NIH HHS; CA-14051/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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52665-69-7/Calcimycin; 59-46-1/Procaine; 67-68-5/Dimethyl Sulfoxide; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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