| Problems with measurements caused by high concentrations of serum solids. | |
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MedLine Citation:
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PMID: 3301210 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There have been numerous reports of spectrophotometric and volume problems caused by elevated levels of lipids in blood. The offending lipids, primarily triglycerides, not only cause turbidity leading to optical aberrations when added to analytical reagents, but also result in short-sampling errors leading to the measurement of inaccurate volumes of sample. Numerous methods have been developed to clear the lipemia, including ultracentrifugation organic solvent extraction, chemical precipitation and, most recently, enzymic hydrolysis. Although the latter procedures eliminate the optical problems, they do not deal with the volume dilution error created by the triglycerides. In turn, corrective mathematics have been developed to compensate for the inaccurate pipetting caused by the elevated lipids in a sample; however, these empirical calculations are not truly accurate at high concentrations of total lipids. This monograph will describe the problems caused by the presence of elevated lipids and the means available for treating them. |
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Authors:
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J D Artiss; B Zak |
Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Critical reviews in clinical laboratory sciences Volume: 25 ISSN: 1040-8363 ISO Abbreviation: Crit Rev Clin Lab Sci Publication Date: 1987 |
Date Detail:
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Created Date: 1987-09-23 Completed Date: 1987-09-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8914816 Medline TA: Crit Rev Clin Lab Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 19-41 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blood Chemical Analysis* Blood Proteins / analysis Blood Volume Erythrocyte Indices Humans Hyperlipidemias / blood Lipids / blood Spectrophotometry |
| Chemical | |
Reg. No./Substance:
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0/Blood Proteins; 0/Lipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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