| Problems associated with reporter assays in RNAi studies. | |
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MedLine Citation:
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PMID: 19617709 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Reporter assays represent a facile tool for studies of RNA interference and are routinely used in functional tests or mechanistic studies of siRNAs and miRNAs. Unfortunately some reporter assays were developed without careful consideration when they were adopted from studies of transcriptional regulation using promoter-reporter gene fusions. Here we report on some problems in the use of reporter systems for studies of RNA interference and make some suggestions for avoiding such problems in future studies. |
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Authors:
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Guihua Sun; John J Rossi |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-09-05 |
Journal Detail:
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Title: RNA biology Volume: 6 ISSN: 1555-8584 ISO Abbreviation: RNA Biol Publication Date: 2009 Sep-Oct |
Date Detail:
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Created Date: 2009-11-06 Completed Date: 2010-02-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101235328 Medline TA: RNA Biol Country: United States |
Other Details:
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Languages: eng Pagination: 406-11 Citation Subset: IM |
Affiliation:
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Graduate School of Biological Science, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Biological Assay / methods* Cell Line Genes, Reporter / genetics* Genetic Vectors / genetics Humans MicroRNAs / chemistry, genetics Molecular Sequence Data Nucleic Acid Conformation RNA Interference* |
| Grant Support | |
ID/Acronym/Agency:
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AI29329/AI/NIAID NIH HHS; HL07470/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/MicroRNAs |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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