| Probing the redox activity of T-lymphocytes deposited at electrode surfaces with voltammetric methods. | |
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MedLine Citation:
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PMID: 18076353 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Reactive oxygen species and redox signaling play an important role in the regulation of many vital biological processes. However, they are also tightly connected with many pathological conditions. The detection and evaluation of these signaling events are very often accompanied with great difficulties. In this article, we describe the development of a novel electrochemically-based technique for monitoring the cellular redox state. METHODS AND RESULTS: T-cells were attached on the surface of a working electrode, which was modified with 2-palmitoylhydroquinone as a redox mediator. Using cyclic voltammetry, we were able to indirectly (via the redox mediator) monitor an electron transport from the cells towards the working electrode, which enabled us to evaluate the redox activity of the cells. CONCLUSIONS: This new technique is rather simple and sensitive and may be used in the future as a valid diagnostic procedure in various branches of biomedical science. |
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Authors:
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Ivan Bogeski; Valentin Mirceski; Markus Hoth |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical chemistry and laboratory medicine : CCLM / FESCC Volume: 46 ISSN: 1434-6621 ISO Abbreviation: Clin. Chem. Lab. Med. Publication Date: 2008 |
Date Detail:
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Created Date: 2008-03-07 Completed Date: 2008-05-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9806306 Medline TA: Clin Chem Lab Med Country: Germany |
Other Details:
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Languages: eng Pagination: 197-203 Citation Subset: IM |
Affiliation:
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Department of Physiology, Saarland University, Homburg, Germany. ivan.bogeski@uks.eu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Electrochemistry
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instrumentation,
methods* Electrodes* Humans Jurkat Cells Oxidation-Reduction T-Lymphocytes / immunology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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