Document Detail

Probing of photosynthetic reactions in four phytoplanktonic algae with a PEA fluorometer.
MedLine Citation:
PMID:  19731073     Owner:  NLM     Status:  MEDLINE    
High-resolution light-induced kinetics of chlorophyll fluorescence (OJIP transients) were recorded and analyzed in cultures of diatoms (Thalassiosira weissflogii, Chaetoceros mulleri) and dinoflagellates (Amphidinium carterae, Prorocentrum minimum). Fluorescence transients showed the rapid exponential initial rise from the point O indicating low connectivity between PS II units and high absorption cross-section of PS II antenna. Dark-adapted dinoflagellates revealed capability to maintain the PS I-mediated re-oxidation of the PQ pool at the exposure to strong actinic light that may lead to the underestimation of F(M) value. In OJIP transients recorded in phytoplanktonic algae the fluorescence yield at the point O exceeded F(O) level because Q(A) has been already partly reduced at 50 micros after the illumination onset. PEA was also employed to study the recovery of photosynthetic reactions in T. weissflogii during incubation of nitrogen starved cells in N-replete medium. N limitation caused the impairment of electron transport between Q(A) and PQs, accumulation of closed PS II centers, and the reduced ability to generate transmembrane DeltapH upon illumination, almost fully restored during the recovery period. The recovered cells showed much higher values of NPQ than control ones suggesting maximization of photoprotection mechanisms in the population with a 'stress history.'
T K Antal; D N Matorin; L V Ilyash; A A Volgusheva; V Osipov; I V Konyuhov; T E Krendeleva; A B Rubin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Photosynthesis research     Volume:  102     ISSN:  1573-5079     ISO Abbreviation:  Photosyn. Res.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-06     Completed Date:  2010-02-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100954728     Medline TA:  Photosynth Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  67-76     Citation Subset:  IM    
Faculty of Biology, Moscow State University, Moscow, Russia.
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MeSH Terms
Algae / physiology*
Chlorophyll / metabolism
Fluorometry / instrumentation*
Nitrogen / deficiency
Photosynthesis / physiology*
Phytoplankton / physiology*
Reg. No./Substance:
1406-65-1/Chlorophyll; 7727-37-9/Nitrogen

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