| Probing nucleic acid-ion interactions with buffer exchange-atomic emission spectroscopy. | |
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MedLine Citation:
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PMID: 20946799 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ion atmosphere of nucleic acids directly affects measured biochemical and biophysical properties. However, study of the ion atmosphere is difficult due to its diffuse and dynamic nature. Standard techniques available have significant limitations in sensitivity, specificity, and directness of the assays. Buffer exchange-atomic emission spectroscopy (BE-AES) was developed to overcome many of the limitations of previously available techniques. This technique can provide a complete accounting of all ions constituting the ionic atmosphere of a nucleic acid at thermodynamic equilibrium. Although initially developed for the study of the ion atmosphere of nucleic acids, BE-AES has also been applied to study site-bound ions in RNA and protein. |
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Authors:
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Max Greenfeld; Daniel Herschlag |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Methods in enzymology Volume: 469 ISSN: 1557-7988 ISO Abbreviation: Meth. Enzymol. Publication Date: 2009 |
Date Detail:
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Created Date: 2010-10-15 Completed Date: 2011-01-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0212271 Medline TA: Methods Enzymol Country: United States |
Other Details:
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Languages: eng Pagination: 375-89 Citation Subset: IM |
Copyright Information:
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Copyright © 2009 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Chemical Engineering and Biochemistry, Stanford University, Stanford, California, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Buffers Ions / chemistry* Nucleic Acids / chemistry* Spectrophotometry, Atomic / methods* |
| Grant Support | |
ID/Acronym/Agency:
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GM49243/GM/NIGMS NIH HHS; P01-GM-66275/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Buffers; 0/Ions; 0/Nucleic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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