| Probing the membrane potential of living cells by dielectric spectroscopy. | |
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MedLine Citation:
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PMID: 19579022 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this paper we demonstrate a quantitative way to measure the membrane potential of live cells by dielectric spectroscopy. We also show that the values of the membrane potential obtained using our technique are in good agreement with those obtained using traditional methods-voltage-sensitive dyes. The membrane potential is determined by fitting the experimental dielectric dispersion curves with the dispersion curves obtained from a theoretical model. Variations in the membrane potential were induced by modifying the concentration of potassium chloride in the solution of the cell suspension in the presence of valinomycin. For exemplification of the method, E. coli was chosen for our experiments. |
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Authors:
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Corina Bot; C Prodan |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-07-05 |
Journal Detail:
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Title: European biophysics journal : EBJ Volume: 38 ISSN: 1432-1017 ISO Abbreviation: Eur. Biophys. J. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-10-02 Completed Date: 2009-12-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8409413 Medline TA: Eur Biophys J Country: Germany |
Other Details:
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Languages: eng Pagination: 1049-59 Citation Subset: IM |
Affiliation:
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Physics Department, New Jersey Institute of Technology, Newark, NJ 07102, USA. corina.bot@njit.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cytological Techniques
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methods* Electric Impedance Escherichia coli / physiology* Membrane Potentials / physiology* Spectrum Analysis / methods* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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