Document Detail

Probing the function of Drosophila melanogaster accessory glands by directed cell ablation.
MedLine Citation:
PMID:  8367469     Owner:  NLM     Status:  MEDLINE    
The female Drosophila melanogaster fly undergoes behavioral changes after mating, including an increase in egg laying and an avoidance of remating. Accessory-gland products elicit these changes transiently when introduced into unmated female flies. We report here the generation and phenotype of flies that lack functional accessory-gland main cells as a consequence of genetically directed delivery of diphtheria toxin subunit A to those cells. Only main-cell secretions are essential for the short-term inhibition to remating; no other products of the genital tract can replace their function. Long-term inhibition to remating depends only on the storage of sperm in the female. Both sperm and main-cell secretions have roles in the increase of egg laying by the mated female. In addition to full-strength diphtheria toxin, we used low-activity toxins to kill only those cells that express toxin at high levels. These transgenic strains that express diphtheria toxins of different strengths in accessory-gland main cells will be useful in further defining the role of these cells.
J M Kalb; A J DiBenedetto; M F Wolfner
Related Documents :
8180689 - A new assay for the invasive adenylate cyclase toxin of bordetella pertussis based on i...
12706629 - Cloning of a heliothis virescens 110 kda aminopeptidase n and expression in drosophila ...
2169699 - Calmodulin-dependent enzymes as a target of staphylococcal enterotoxin a.
3496269 - Cap formation in a b-lymphocyte cell line is inhibited by pertussis toxin and phorbol e...
15635689 - Ochratoxin a at nanomolar concentrations: a signal modulator in renal cells.
2988809 - Nad and the synthesis of (adp-ribose)n in a human cell strain (46br) hypersensitive to ...
2519879 - Kinetics of cell replication in the uterine cervix. viii. quantitation of chronologic e...
22902639 - Uhrf2, another e3 ubiquitin ligase for p53.
6331129 - Defective replication of porcine transmissible gastroenteritis virus in a continuous ce...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  90     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1993-10-05     Completed Date:  1993-10-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  8093-7     Citation Subset:  IM    
Section of Genetics and Development, Cornell University, Ithaca, NY 14853-2703.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Crosses, Genetic
Diphtheria Toxin / biosynthesis
Drosophila melanogaster / cytology,  genetics,  physiology*
Promoter Regions, Genetic
Protein Biosynthesis
Restriction Mapping
Sebaceous Glands / cytology,  physiology
Spermatozoa / physiology
Testis / physiology
Reg. No./Substance:
0/Diphtheria Toxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Targeting and germ-line transmission of a null mutation at the metallothionein I and II loci in mous...
Next Document:  Binding of human apolipoprotein E to synthetic amyloid beta peptide: isoform-specific effects and im...