Document Detail

Probing cell chemistry with time-of-flight secondary ion mass spectrometry: development and exploitation of instrumentation for studies of frozen-hydrated biological material.
MedLine Citation:
PMID:  14515313     Owner:  NLM     Status:  MEDLINE    
Imaging static secondary ion mass spectrometry (SIMS) offers a powerful method of obtaining molecular information from biological systems with good spatial resolution. However, the technique needs further development to make it suitable for routine analysis of cells. We report here the development of a new freeze-facture device to facilitate the manipulation and analysis of biological cell material, with the cell chemistry preserved intact by rapid freezing. We illustrate performance characteristics with high-contrast images of freeze-fractured, frozen-hydrated liposomes with the drug clofazamine constrained within the lipid bilayer providing a marker to determine the fracture plane across the liposome structure. By monitoring and imaging clofazamine on the surface of yeast cells in the frozen-hydrated state, and demonstrating its absence within molecular information from a cell fractured to reveal the cell ultrastructure, we demonstrate that the molecule does not penetrate the cell wall.
Ben Cliff; Nicholas Lockyer; Harald Jungnickel; Gill Stephens; John C Vickerman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  17     ISSN:  0951-4198     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2003  
Date Detail:
Created Date:  2003-09-29     Completed Date:  2004-01-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  2163-7     Citation Subset:  IM    
Copyright Information:
Copyright 2003 John Wiley & Sons, Ltd.
Surface Analysis Research Centre, Department of Chemistry, UMIST, Manchester M60 1QD, UK.
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MeSH Terms
Candida glabrata / cytology*
Cell Wall / metabolism
Clofazimine / analysis
Freeze Fracturing
Liposomes / chemistry*
Spectrometry, Mass, Secondary Ion / instrumentation*,  methods*
Reg. No./Substance:
0/Liposomes; 2030-63-9/Clofazimine

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