Document Detail

Probing cytotoxicity of gadolinium hydroxide nanostructures.
MedLine Citation:
PMID:  20218658     Owner:  NLM     Status:  MEDLINE    
Gadolinium hydroxide, Gd(OH)(3), nanostructures were examined for their possible use in imaging and tracking of cells and tissues by investigating their cellular interactions and cytotoxic behaviors. For this purpose, Gd(OH)(3) nanorods (length, several hundred nanometers; diameter, approximately 40 nm) and spherical nanoparticles (average diameter, <10 nm) were synthesized by solvothermal decomposition of gadolinium containing molecular precursors. After comprehensive characterization of material properties, human colon adenocarcinoma (Caco2) and human lung epithelial (A549) cells were incubated with Gd(OH)(3) nanostructures in concentrations up to 900 microg/mL to perform cytotoxicity assays (BrdU (5-bromo-2'-deoxyuridine), WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzol-disulfonate)) and living/dead staining. As observed in all in vitro assays, the investigated Gd(OH)(3) nanostructures do not induce any significant cytotoxic effect, making them promising candidates for a new class of contrast agents, which may overcome the limitations of organic stains such as photobleaching and single usage.
Eva Hemmer; Yvonne Kohl; Victoria Colquhoun; Hagen Thielecke; Kohei Soga; Sanjay Mathur
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journal of physical chemistry. B     Volume:  114     ISSN:  1520-5207     ISO Abbreviation:  J Phys Chem B     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-25     Completed Date:  2010-07-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101157530     Medline TA:  J Phys Chem B     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4358-65     Citation Subset:  IM    
Department of Materials Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510 Chiba, Japan.
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MeSH Terms
Cell Line, Tumor
Cell Survival / drug effects
Gadolinium / chemistry,  toxicity*
Reg. No./Substance:

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