| Probes and techniques for sperm evaluation by flow cytometry. | |
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MedLine Citation:
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PMID: 20591067 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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CONTENTS: Flow cytometry has become an important technique in sperm evaluation and is increasingly used both for routine assessment and for research in veterinary science. We have revised the literature, describing fluorescent probes that have been used for analysing spermatozoa by flow cytometry, regarding: viability, acrosomal status, capacitation, mitochondrial status, apoptotic markers, oxidative stress markers, DNA damage, sperm counting and sperm sizing. Details and problems of some techniques are reviewed, with special attention to the occurrence of non-sperm particles in the samples ('debris'). New and promising aspects of flow cytometry, such as sperm sorting using viability markers as selection criteria, are highlighted. The relationship between flow cytometry analyses and fertility and their future improvements are considered. |
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Authors:
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F Martínez-Pastor; M Mata-Campuzano; M Alvarez-Rodríguez; M Alvarez; L Anel; P de Paz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: Reproduction in domestic animals = Zuchthygiene Volume: 45 Suppl 2 ISSN: 1439-0531 ISO Abbreviation: Reprod. Domest. Anim. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-07-01 Completed Date: 2010-10-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9015668 Medline TA: Reprod Domest Anim Country: Germany |
Other Details:
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Languages: eng Pagination: 67-78 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology Cell Biology, ITRA-ULE, INDEGSAL, University of León, León, Spain. fmarp@unileon.es |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acrosome
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physiology Animals Apoptosis Cell Size Cell Survival DNA Damage Fertilization in Vitro / veterinary Flow Cytometry / methods, veterinary* Fluorescent Dyes Male Mitochondria / physiology Oxidative Stress Sperm Capacitation Sperm Count Spermatozoa / physiology*, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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