Document Detail

Probe pressure effects on human skin diffuse reflectance and fluorescence spectroscopy measurements.
MedLine Citation:
PMID:  21280899     Owner:  NLM     Status:  MEDLINE    
Diffuse reflectance and fluorescence spectroscopy are popular research techniques for noninvasive disease diagnostics. Most systems include an optical fiber probe that transmits and collects optical spectra in contact with the suspected lesion. The purpose of this study is to investigate probe pressure effects on human skin spectroscopic measurements. We conduct an in-vivo experiment on human skin tissue to study the short-term (<2 s) and long-term (>30 s) effects of probe pressure on diffuse reflectance and fluorescence measurements. Short-term light probe pressure (P0<9 mN∕mm2) effects are within 0 ± 10% on all physiological properties extracted from diffuse reflectance and fluorescence measurements, and less than 0±5% for diagnostically significant physiological properties. Absorption decreases with site-specific variations due to blood being compressed out of the sampled volume. Reduced scattering coefficient variation is site specific. Intrinsic fluorescence shows a large standard error, although no specific pressure-related trend is observed. Differences in tissue structure and morphology contribute to site-specific probe pressure effects. Therefore, the effects of pressure can be minimized when the pressure is small and applied for a short amount of time; however, long-term and large pressures induce significant distortions in measured spectra.
Liang Lim; Brandon Nichols; Narasimhan Rajaram; James W Tunnell
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  16     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:    2011 Jan-Feb
Date Detail:
Created Date:  2011-02-01     Completed Date:  2011-05-31     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  011012     Citation Subset:  IM    
The University of Texas at Austin, Department of Biomedical Engineering, 1 University Station C0800, Austin, Texas 78712, USA.
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MeSH Terms
Equipment Design
Equipment Failure Analysis
Photometry / instrumentation*
Reproducibility of Results
Sensitivity and Specificity
Skin / chemistry*
Spectrometry, Fluorescence / instrumentation*
Young Adult
Grant Support

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