Document Detail


Proapoptotic effect of metalloproteinase 9 secreted by trophoblasts on endothelial cells.
MedLine Citation:
PMID:  21159039     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: During normal placentation, the uterine spiral arteries are remodeled to form high-flow, low-resistance vessels, which is necessary to meet the increasing demands of the fetus for blood and oxygen. Remodeling of uterine arteries is mediated by extravillous trophoblasts, which penetrate the arterial walls and induce the endothelial apoptosis. But the mechanism of endothelial apoptosis is not yet fully understood. In this study, we investigate the involvement of matrix metalloproteinase 9 (MMP-9) in trophoblast-induced endothelial apoptosis.
METHODS: MMP-9 small interfering RNA (siRNA) was transfected into extravillous trophoblasts (TEV-1) by lipofectamine. After transfection, the levels of MMP-9 and Fas Ligand (FasL) mRNA were assessed using real-time polymerase chain reaction (PCR), and the intracellular or extracellular levels of these proteins were measured using enzyme-linked immunosorbent assay. Then, trophoblasts transfected with MMP-9 siRNA or control siRNA were co-cultured with endothelial cells, respectively, in a transwell system. The apoptosis of endothelial cells was detected with flow cytometry.
RESULTS: MMP-9 mRNA was significantly decreased in MMP-9 siRNA-treated cells as compared with the control. However, FasL mRNA appeared unaffected. After transfection with MMP-9 siRNA, the intracellular and extracellular MMP-9 proteins were both efficiently decreased, as the soluble FasL protein, while the intracellular FasL protein was increased. Trophoblasts transfected with MMP-9 siRNA led to significant inhibition of endothelial apoptosis as compared with the control.
CONCLUSIONS: Our results suggest that MMP-9 secreted by trophoblasts contributes to endothelial apoptosis via extracellular release of soluble FasL.
Authors:
Jingjing Xu; Haiyi Liu; Yuanyuan Wu; Xun Gong; Qiong Zhou; Fuyuan Qiao
Related Documents :
21318419 - Vegf masks bnip3-mediated apoptosis of hypoxic endothelial cells.
21741249 - Arthrinins a-d: novel diterpenoids and further constituents from the sponge derived fun...
21626719 - The effect of astrocytes on the induction of barrier properties in aortic endothelial c...
21967109 - Cd147-targeting sirna inhibits cell-matrix adhesion of human malignant melanoma cells b...
21930389 - The mechanism of adhesion and germination in the carpospores of porphyra spiralis var. ...
21955679 - Novel natural product-based cinnamates and their thio and thiono analogs as potent inhi...
10391499 - Immunofluorescence detection of cadherins in mouse tooth germs during root development.
23154239 - Stimulation of non-neuronal muscarinic receptors enhances chemerin/chemr23 system in dy...
2712859 - Densitometric measurement of increased endothelial permeability in arteriosclerotic pla...
Publication Detail:
Type:  Journal Article     Date:  2010-12-16
Journal Detail:
Title:  The journal of obstetrics and gynaecology research     Volume:  37     ISSN:  1447-0756     ISO Abbreviation:  J. Obstet. Gynaecol. Res.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-07     Completed Date:  2011-10-28     Revised Date:  2012-01-19    
Medline Journal Info:
Nlm Unique ID:  9612761     Medline TA:  J Obstet Gynaecol Res     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  187-94     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors. Journal of Obstetrics and Gynaecology Research © 2010 Japan Society of Obstetrics and Gynecology.
Affiliation:
Department of Obstetrics-Gynecology, Tongji Hospital, Huazhong University of Science and Technology, Hubei, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Apoptosis / physiology*
Cell Line
Endothelial Cells / cytology*
Fas Ligand Protein / genetics,  physiology*
Female
Gene Expression
Humans
Matrix Metalloproteinase 9 / genetics,  physiology*
Placentation
Pregnancy
RNA, Messenger / analysis
RNA, Small Interfering / genetics
Reverse Transcriptase Polymerase Chain Reaction
Transfection
Trophoblasts / chemistry,  enzymology*
Chemical
Reg. No./Substance:
0/Fas Ligand Protein; 0/RNA, Messenger; 0/RNA, Small Interfering; EC 3.4.24.35/Matrix Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Association between pre-pregnancy body mass index and socioeconomic status and impact on pregnancy o...
Next Document:  Neurological complications without eclampsia during pregnancy in Turkey.