| Pro-oxidant and cytotoxic activities of atractylenolide I in human promyeloleukemic HL-60 cells. | |
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MedLine Citation:
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PMID: 16624472 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The dried rhizome of Bai Zhu (Atractylodes ovata) is widely used as a Chinese herbal medicine. Two sesquiterpenolides of similar structures (atractylenolide I, AT-I; atractylenolide III, AT-III) were isolated from dried rhizome of Atractylodes ovata. Incubation of AT-I with recombinant human Cu,Zn-superoxide dismutase (rhCu,Zn-SOD) resulted in rhCu,Zn-SOD fragmentations and Zn releases. However, these were not observed in the AT-III reaction. The AT-1 showed dose-dependent cytotoxic activities (7.5, 15, and 30 microg/ml) on the human promyeloleukemic HL-60 cells while AT-III did not, and the IC50 of the former being 10.6 microg/ml (corresponding to 46 microM) on 12 h-treated cells. The results of DNA ladder and DNA contents in sub-G1 type revealed that AT-I induced apoptosis in human promyeloleukemic HL-60 cells. The cytotoxic and pharmacological mechanisms of AT-I against human promyeloleukemic HL-60 cells was investigated. The AT-I appeared to exhibit both pro-oxidant and antioxidant properties after an ESR spectrometer was used to detect hydroxyl radical productions in vitro and flow cytometry to detect intracellular ROS productions in AT-I treated cells. The AT-1 also showed dose-dependent Cu,Zn-SOD inhibitory activity in HL-60 cells treated for 12 h, confirmed by activity and immune stainings. However, catalase, Mn-SOD, and glutathione peroxidase did not apparently change activities under the same treatments. The addition of commercial rhCu,Zn-SOD (25-100 U/mL) to the AT-I-treated HL-60 cells (15 microg/ml) resulted in significant differences (p<0.01) and could reduce the AT-I cytotoxicity from 78% to 28% on HL-60 cells. It was proposed that the AT-I might work via Cu,Zn-SOD inhibition in HL-60 cells to induce apoptosis and bring about cytotoxicity. |
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Authors:
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Ching-Chiung Wang; Shyr-Yi Lin; Huey-Chuan Cheng; Wen-Chi Hou |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-02-28 |
Journal Detail:
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Title: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association Volume: 44 ISSN: 0278-6915 ISO Abbreviation: Food Chem. Toxicol. Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-06-23 Completed Date: 2006-09-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8207483 Medline TA: Food Chem Toxicol Country: England |
Other Details:
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Languages: eng Pagination: 1308-15 Citation Subset: IM |
Affiliation:
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Graduate Institute of Pharmacognosy, Taipei Medical University Hospital, No. 250, Wu-Hsing Street, Taipei 110, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects Asteraceae / chemistry* Cell Survival / drug effects, physiology DNA Fragmentation / drug effects, physiology Drug Interactions Electron Spin Resonance Spectroscopy Flow Cytometry Free Radical Scavengers / pharmacology HL-60 Cells Humans Hydrogen Peroxide Hydroxyl Radical / metabolism Inhibitory Concentration 50 Iron Lactones / pharmacology* Peroxides / metabolism Recombinant Proteins / pharmacology Rhizome / chemistry Sesquiterpenes / pharmacology* Superoxide Dismutase / antagonists & inhibitors, metabolism*, pharmacology Zinc / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Fenton's reagent; 0/Free Radical Scavengers; 0/Lactones; 0/Peroxides; 0/Recombinant Proteins; 0/Sesquiterpenes; 0/atractylenolide I; 0/atractylenolide III; 3352-57-6/Hydroxyl Radical; 7439-89-6/Iron; 7440-66-6/Zinc; 7722-84-1/Hydrogen Peroxide; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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