Document Detail

Pro-inflammatory effects of Burkholderia cepacia on cystic fibrosis respiratory epithelium.
MedLine Citation:
PMID:  14522463     Owner:  NLM     Status:  MEDLINE    
Burkholderia cepacia causes pulmonary infection with high mortality in cystic fibrosis (CF) patients which is likely to involve interaction with respiratory epithelium. In this study the pro-inflammatory properties of B. cepacia were examined using a range of respiratory epithelial cell lines. B. cepacia and cell-free culture supernatants were used to stimulate cell lines with (SigmaCFTE29o- and IB3) and without (A549) the CF transmembrane conductance regulator mutation (CFTR), together with corrected cell lines (C38 and S9). Interleukin (IL)-6 and IL-8, but not GM-CSF or IL-1beta, were released from all the cell lines whereas PGE(2) (prostaglandin E(2)) was released from the A549, IB3 and S9 cell lines only. Nuclear factor (NF)-kappaB activation preceded cytokine release and suppression of NF-kappaB activity diminished cytokine release. These studies indicated that B. cepacia secretory products are potent pro-inflammatory agents for respiratory epithelium and suggest functional CFTR is not required for cytokine or prostanoid responses.
Joshua Fink; James H Steer; David A Joyce; Andrew S McWilliam; Geoffrey A Stewart
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS immunology and medical microbiology     Volume:  38     ISSN:  0928-8244     ISO Abbreviation:  FEMS Immunol. Med. Microbiol.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-02     Completed Date:  2003-12-12     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9315554     Medline TA:  FEMS Immunol Med Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  273-82     Citation Subset:  IM    
School of Biomedical and Chemical Sciences, University of Western Australia, Nedlands, WA 6009, Australia.
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MeSH Terms
Burkholderia cepacia / immunology,  pathogenicity*
Cell Line
Cell Line, Tumor
Cystic Fibrosis / immunology,  metabolism,  microbiology*
Cystic Fibrosis Transmembrane Conductance Regulator / genetics,  physiology
Cytokines / metabolism*
Dinoprostone / metabolism*
Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
Interleukin-1 / metabolism
Interleukin-6 / metabolism
Interleukin-8 / metabolism
NF-kappa B / metabolism
Prostaglandin-Endoperoxide Synthases / metabolism
Respiratory Mucosa / immunology,  metabolism*,  microbiology*
Transcriptional Activation
Reg. No./Substance:
0/CFTR protein, human; 0/Cytokines; 0/Interleukin-1; 0/Interleukin-6; 0/Interleukin-8; 0/NF-kappa B; 126880-72-6/Cystic Fibrosis Transmembrane Conductance Regulator; 363-24-6/Dinoprostone; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor; EC Synthases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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