| Pro-inflammatory effects of Burkholderia cepacia on cystic fibrosis respiratory epithelium. | |
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MedLine Citation:
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PMID: 14522463 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Burkholderia cepacia causes pulmonary infection with high mortality in cystic fibrosis (CF) patients which is likely to involve interaction with respiratory epithelium. In this study the pro-inflammatory properties of B. cepacia were examined using a range of respiratory epithelial cell lines. B. cepacia and cell-free culture supernatants were used to stimulate cell lines with (SigmaCFTE29o- and IB3) and without (A549) the CF transmembrane conductance regulator mutation (CFTR), together with corrected cell lines (C38 and S9). Interleukin (IL)-6 and IL-8, but not GM-CSF or IL-1beta, were released from all the cell lines whereas PGE(2) (prostaglandin E(2)) was released from the A549, IB3 and S9 cell lines only. Nuclear factor (NF)-kappaB activation preceded cytokine release and suppression of NF-kappaB activity diminished cytokine release. These studies indicated that B. cepacia secretory products are potent pro-inflammatory agents for respiratory epithelium and suggest functional CFTR is not required for cytokine or prostanoid responses. |
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Authors:
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Joshua Fink; James H Steer; David A Joyce; Andrew S McWilliam; Geoffrey A Stewart |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEMS immunology and medical microbiology Volume: 38 ISSN: 0928-8244 ISO Abbreviation: FEMS Immunol. Med. Microbiol. Publication Date: 2003 Oct |
Date Detail:
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Created Date: 2003-10-02 Completed Date: 2003-12-12 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9315554 Medline TA: FEMS Immunol Med Microbiol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 273-82 Citation Subset: IM |
Affiliation:
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School of Biomedical and Chemical Sciences, University of Western Australia, Nedlands, WA 6009, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Burkholderia cepacia
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immunology,
pathogenicity* Cell Line Cell Line, Tumor Cystic Fibrosis / immunology, metabolism, microbiology* Cystic Fibrosis Transmembrane Conductance Regulator / genetics, physiology Cytokines / metabolism* Dinoprostone / metabolism* Granulocyte-Macrophage Colony-Stimulating Factor / metabolism Humans Inflammation* Interleukin-1 / metabolism Interleukin-6 / metabolism Interleukin-8 / metabolism Mutation NF-kappa B / metabolism Prostaglandin-Endoperoxide Synthases / metabolism Respiratory Mucosa / immunology, metabolism*, microbiology* Transcriptional Activation |
| Chemical | |
Reg. No./Substance:
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0/CFTR protein, human; 0/Cytokines; 0/Interleukin-1; 0/Interleukin-6; 0/Interleukin-8; 0/NF-kappa B; 126880-72-6/Cystic Fibrosis Transmembrane Conductance Regulator; 363-24-6/Dinoprostone; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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