| Pro-gliogenic effect of IL-1alpha in the differentiation of embryonic neural precursor cells in vitro. | |
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MedLine Citation:
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PMID: 20236219 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Inflammation is regarded as a main obstacle to brain regeneration. Major detrimental effects are attributed to microglial/macrophagic products, such as TNF-alpha and interleukin (IL)-6. The role of cytokines of the IL-1 family, particularly of IL-1alpha, in the modulation of neural precursor cell (NPC) properties is less characterized. IL-1alpha is one of the most abundant cytokines released upon acute stimulation of microglia with lipopolysaccharide and is down-regulated upon chronic stimulation. As we recently demonstrated, acutely activated microglia reduces NPC survival, prevent neuronal differentiation and promote glial differentiation. Chronically activated microglia are instead permissive to NPC survival and neuronal differentiation, and less effective in promoting astrocytic differentiation. We thus investigated whether IL-1alpha could contribute to the effects of acutely activated microglia on NPC. We found that NPC express functional IL-1 receptors and that exposure to recombinant IL-1alpha strongly enhances NPC differentiation into astrocytes, without affecting cell viability and neuronal differentiation. In the same conditions, recombinant IL-1beta has pro-gliogenic effects at concentrations 10-fold higher than those found in activated microglial conditioned media. Interestingly, immunodepletion of IL-1alpha in activated microglial conditioned media fails to revert microglial pro-gliogenic action and slightly enhances neuronal differentiation, revealing that other microglial-derived factors contribute to the modulation of NPC properties. |
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Authors:
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Maria Antonietta Ajmone-Cat; Emanuele Cacci; Ylenia Ragazzoni; Luisa Minghetti; Stefano Biagioni |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-04 |
Journal Detail:
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Title: Journal of neurochemistry Volume: 113 ISSN: 1471-4159 ISO Abbreviation: J. Neurochem. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-27 Completed Date: 2010-06-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: England |
Other Details:
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Languages: eng Pagination: 1060-72 Citation Subset: IM |
Affiliation:
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Department of Cell Biology and Neuroscience, Istituto Superiore di Sanit?, Rome, Italy. ajcat@iss.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Astrocytes / drug effects, metabolism Cell Differentiation / drug effects*, physiology Cell Lineage / drug effects*, physiology Cells, Cultured Coculture Techniques Culture Media, Conditioned / pharmacology Cytokines / genetics, metabolism, pharmacology Dose-Response Relationship, Drug Embryonic Stem Cells / cytology, drug effects*, metabolism Encephalitis / metabolism, physiopathology Gliosis / metabolism, physiopathology Interleukin-1alpha / genetics, metabolism, pharmacology* Mice Microglia / drug effects, metabolism Neuroglia / cytology, drug effects*, metabolism Neurons / cytology, drug effects, metabolism Rats Receptors, Interleukin-1 / drug effects, metabolism Recombinant Fusion Proteins / genetics, metabolism, pharmacology Stem Cells / cytology, drug effects*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 0/Cytokines; 0/Interleukin-1alpha; 0/Receptors, Interleukin-1; 0/Recombinant Fusion Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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