| Pro-apoptotic effects of nivalenol and deoxynivalenol trichothecenes in J774A.1 murine macrophages. | |
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MedLine Citation:
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PMID: 19410639 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nivalenol (NIV) and deoxynivalenol (DON) are trichothecenes mycotoxins produced by Fusarium fungi that occur in cereal grains alone or in combination. Several studies have shown that exposure to high concentrations of these mycotoxins resulted in decreased cell proliferation; however, the molecular mechanism underlying their activities are still partially known. In this study, we evaluated the effects of NIV and DON, alone and in combination, on J7741.A macrophages viability. The results of the current study show that both NIV and DON (10-100 microM) significantly stimulate apoptosis in J774A.1 macrophages in a concentration-dependent manner; in particular, NIV results a stronger pro-apoptotic effect than DON on cultured J774A.1 murine macrophages. No interactive effects were observed by exposing J774A.1 cells to both NIV and DON simultaneously. Pro-apoptotic activity induced by both mycotoxins seems to be essentially mediated by caspase-3 and is associated with a cell cycle blocking in G0/G1 phase. Moreover, our results show that NIV and DON are able to influence apoptotic pathway by ERK, pro-apoptotic protein Bax, caspase-3 and poly-ADP-ribose synthase (PARP), DNA repairing enzyme. |
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Authors:
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Stefania Marzocco; Rosario Russo; Giuseppe Bianco; Giuseppina Autore; Lorella Severino |
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Publication Detail:
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Type: Journal Article Date: 2009-05-03 |
Journal Detail:
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Title: Toxicology letters Volume: 189 ISSN: 1879-3169 ISO Abbreviation: Toxicol. Lett. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-06-22 Completed Date: 2009-07-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7709027 Medline TA: Toxicol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 21-6 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutical Sciences, School of Pharmacy, University of Salerno, Fisciano (SA) 84084, Italy. smarzocco@unisa.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Blotting, Western Cell Cycle / drug effects Cell Line Cell Proliferation / drug effects Cell Survival / drug effects Dose-Response Relationship, Drug Drug Interactions Macrophages / drug effects*, enzymology, immunology, metabolism Mice Mycotoxins / toxicity* Trichothecenes / toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Mycotoxins; 0/Trichothecenes; 23282-20-4/nivalenol; 51481-10-8/deoxynivalenol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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