Document Detail


Prion protein (PrP) is not involved in the pathogenesis of spongiform encephalopathy in zitter rats.
MedLine Citation:
PMID:  7909925     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to elucidate the relationship between the prion protein (PrP) structure and the development of spongiform encephalopathy in zitter rats, we analyzed the nucleotide sequences and restriction fragment length variation (RFLV) of the Prn gene encoding PrP in zitter rats and inbred SD/J rats as a control. Prn genes from two strains had identical nucleotide sequences in their coding sequences. Obvious RFLV on the locus was not detected in zitter rats by a Southern blot hybridization. Consistently, zitter rat brains express the normal cellular PrP (PrPC), but do not accumulate the protease-resistant modified isoform (PrPSC). These results indicate that PrP is not involved in the pathogenesis of spongiform encephalopathy in zitter rats.
Authors:
H Gomi; T Ikeda; T Kunieda; S Itohara; S B Prusiner; K Yamanouchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  166     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-06-09     Completed Date:  1994-06-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  171-4     Citation Subset:  IM    
Affiliation:
Institute for Virus Research, Kyoto University, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/S69654
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Blotting, Southern
Blotting, Western
Brain / pathology
Molecular Sequence Data
Open Reading Frames
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Prion Diseases / genetics,  metabolism*
Prions / metabolism*
Rats
Rats, Mutant Strains
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/Prions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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