Document Detail

Prion-inducing domain of yeast Ure2p and protease resistance of Ure2p in prion-containing cells.
MedLine Citation:
PMID:  7569955     Owner:  NLM     Status:  MEDLINE    
The genetic properties of the [URE3] non-Mendelian element of Saccharomyces cerevisiae suggest that it is a prion (infectious protein) form of Ure2p, a regulator of nitrogen catabolism. In extracts from [URE3] strains, Ure2p was partially resistant to proteinase K compared with Ure2p from wild-type extracts. Overexpression of Ure2p in wild-type strains induced a 20- to 200-fold increase in the frequency with which [URE3] arose. Overexpression of just the amino-terminal 65 residues of Ure2p increased the frequency of [URE3] induction 6000-fold. Without this "prion-inducing domain" the carboxyl-terminal domain performed the nitrogen regulation function of Ure2p, but could not be changed to the [URE3] prion state. Thus, this domain induced the prion state in trans, whereas in cis it conferred susceptibility of the adjoining nitrogen regulatory domain to prion infections.
D C Masison; R B Wickner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  270     ISSN:  0036-8075     ISO Abbreviation:  Science     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1995-11-07     Completed Date:  1995-11-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  93-5     Citation Subset:  IM    
Section on Genetics of Simple Eukaryotes, National Institute of Diabetes, Digestive and Kidney Disease, National Institutes of Health, Bethesda, MD 20892-0830, USA.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Endopeptidase K
Fungal Proteins / chemistry,  genetics*,  metabolism
Genes, Fungal
Genetic Complementation Test
Glutathione Peroxidase
Molecular Sequence Data
Nitrogen / metabolism
Prions / genetics*,  metabolism
Promoter Regions, Genetic
Saccharomyces cerevisiae / chemistry,  genetics*,  metabolism
Saccharomyces cerevisiae Proteins*
Sequence Deletion
Serine Endopeptidases / metabolism
Reg. No./Substance:
0/Fungal Proteins; 0/Prions; 0/Saccharomyces cerevisiae Proteins; 7727-37-9/Nitrogen; EC Peroxidase; EC protein, S cerevisiae; EC 3.4.21.-/Serine Endopeptidases; EC K

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