Document Detail


Principles and practical issues for cryopreservation of nerve cells.
MedLine Citation:
PMID:  18158089     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nerve cells isolated from the brain have a number of research and clinical applications, not the least of which is their transplantation to patients with Parkinson's disease. Neural primary and precursor cells of several areas of the brain are potential candidates for transplantation and research. However, supply of suitable tissue is one of the major problems associated with the widespread application of such techniques. The ability to store such tissue for prolonged periods would greatly alleviate this problem. Cryopreservation allows indefinite storage, provided the storage temperature is sufficiently low. Whilst many of the potentially usable cell types have been shown to be capable of surviving cryopreservation to some degree, survival post-thaw needs to be considerably improved. Cryopreservation techniques applied to date are mostly crude and often adopted from those used for unrelated cell types. Studies involving cryopreservation of primary neural cells and stem cells are reviewed, the basic principles of cryopreservation explained and suggestions made for improvements to the low temperature storage of these cells.
Authors:
Sharon J Paynter
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Publication Detail:
Type:  Journal Article; Review     Date:  2007-09-17
Journal Detail:
Title:  Brain research bulletin     Volume:  75     ISSN:  0361-9230     ISO Abbreviation:  Brain Res. Bull.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-25     Completed Date:  2008-04-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7605818     Medline TA:  Brain Res Bull     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-14     Citation Subset:  IM    
Affiliation:
Department of Obstetrics & Gynaecology, Cardiff University, Heath Park, Cardiff CF14 4XN, UK. Paynter@cardiff.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / cytology
Cryopreservation / methods*
Humans
Neurons / classification,  physiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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