Document Detail


Principles of low temperature cell preservation.
MedLine Citation:
PMID:  12878837     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell transplantation is becoming an important technique for treatment of heart failure. Preservation is an integral step in any procedure using cells. There are two primary modes of cell preservation at low temperature, hypothermic preservation at temperatures above freezing and cryogenic preservation at temperatures below freezing. Optimal preservation protocols require a fundamental understanding of the principles involved. This review briefly describes the basic mechanisms of damage during hypothermic and cryogenic preservation and the basic principles for developing optimal protocols for preservation of cells.
Authors:
Boris Rubinsky
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Heart failure reviews     Volume:  8     ISSN:  1382-4147     ISO Abbreviation:  Heart Fail Rev     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-07-24     Completed Date:  2003-12-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9612481     Medline TA:  Heart Fail Rev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  277-84     Citation Subset:  IM    
Affiliation:
Departments of Bioengineering and Mechanical Engineering, University of California at Berkeley, Berkeley CA 94720, USA. rubinsky@me.berkeley.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Physiological Phenomena
Cell Transplantation
Heart Failure / metabolism,  surgery
Humans
Hypothermia, Induced / methods*
Tissue Preservation / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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