| Principles and applications of luminescence spectroscopy. | |
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MedLine Citation:
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PMID: 11310779 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Advances in instrumentation during the last decade as well as the ease with which the technology could be adapted to a wide variety of assay platforms has truly made luminescence spectroscopy the analytical method of choice in several diverse disciplines of life sciences. The primary reasons for its growing popularity are twofold: the use of nonisotopic labels and its exquisite sensitivity. Analyte concentrations as low as 10(-10) to 10(-12) M can be easily detected, while luminometry can detect biological events at concentrations as low as 10(-18) M. This is in contrast to absorption and NMR spectroscopic techniques, which require, respectively, 10(-8) M and 10(-5) M concentrations of the compound of interest. Furthermore, in several variations of this technology, the measurement itself can be nondestructive and noninvasive. Despite the fact that luminescence spectroscopic techniques provide some of the most sensitive and selective analytical methods, they have not yet been widely used in both basic and applied food research. The only exception to this is the growing popularity of the commercially available ATP bioluminescence kits used routinely for monitoring the cleanliness of work surfaces in the food industry. This review describes some of the relevant basic aspects of luminescence, several popular variations of this technology, and their potential uses in food research. |
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Authors:
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S S Deshpande |
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Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Critical reviews in food science and nutrition Volume: 41 ISSN: 1040-8398 ISO Abbreviation: Crit Rev Food Sci Nutr Publication Date: 2001 Mar |
Date Detail:
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Created Date: 2001-04-19 Completed Date: 2001-08-23 Revised Date: 2005-11-16 |
Medline Journal Info:
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Nlm Unique ID: 8914818 Medline TA: Crit Rev Food Sci Nutr Country: United States |
Other Details:
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Languages: eng Pagination: 155-224 Citation Subset: IM |
Affiliation:
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LJL Biosystems, Inc., Sunnyvale, CA 94089, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chemiluminescent Measurements Enzymes / metabolism Flow Cytometry Food Analysis / instrumentation*, methods* Immunologic Techniques Luminescent Measurements* Sensitivity and Specificity Spectrum Analysis / instrumentation*, methods* Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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0/Enzymes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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