Document Detail


Principal component analysis of direct matrix-assisted laser desorption/ionization mass spectrometric data related to metabolites of fatty liver.
MedLine Citation:
PMID:  19367083     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Non-alcoholic steatohepatitis (NASH) is a common liver disease. NASH is characterized by fatty liver, along with inflammation. Most people with NASH are not aware of their condition, even though NASH can lead to hepatic cirrhosis. Several approaches have been tested to clarify the pathology of NASH. However, the mechanism of onset of NASH was not well-defined. In this study, a supervised multivariate analysis (principal component analysis) approach using direct matrix-assisted laser desorption/ionization mass spectrometry (dMALDI-MS) was applied to the analysis of metabolites in starvation-induced fatty liver tissue sections. This approach does not require complex pretreatments. We investigated the characteristic dynamics of metabolites in fatty liver. This approach can be applied to the analysis of human biopsy specimens of fatty liver in future studies.
Authors:
Nobuhiro Zaima; Yumiko Matsuyama; Mitsutoshi Setou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of oleo science     Volume:  58     ISSN:  1347-3352     ISO Abbreviation:  J Oleo Sci     Publication Date:  2009  
Date Detail:
Created Date:  2009-04-15     Completed Date:  2009-07-20     Revised Date:  2009-08-11    
Medline Journal Info:
Nlm Unique ID:  101175339     Medline TA:  J Oleo Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  267-73     Citation Subset:  IM    
Affiliation:
Department of Molecular Anatomy, Molecular Imaging Frontier Research Center, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Japan. zaima@hama-med.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Fatty Liver / metabolism*
Male
Mice
Mice, Inbred C57BL
Multivariate Analysis
Principal Component Analysis / methods*
Reproducibility of Results
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Starvation

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