Document Detail


Primordial follicle activation and follicular development in the juvenile rabbit ovary.
MedLine Citation:
PMID:  16830146     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Of all the stages of mammalian folliculogenesis, the primordial to primary follicle transition is the least understood. In order to gain new insights into this process, we have conducted a comprehensive morphological, morphometric and molecular study of ovarian organisation and early follicle development in the rabbit. The structure of ovaries collected from rabbits aged from 2-12 weeks (a period encompassing primordial follicle formation, activation and the first wave of folliculogenesis in this species) has been analysed by light microscopy and the follicles present have been measured and scored for their developmental stage. To establish useful molecular markers of activation, we have further classified follicles according to their expression of the proliferative marker, proliferating cell nuclear antigen, and the zona pellucida protein, ZPB. The activation of primordial follicles is initiated immediately following their formation in the rabbit ovary and is characterised by oocyte growth, granulosa cell morphogenesis and increased granulosa cell mitosis. Enhanced ZPB protein expression at the oolemma is also associated with follicle activation and development. Few primordial follicles in the juvenile rabbit ovary are lost by atresia, as assessed by the TUNEL assay. The appearance of apoptotic granulosa cells is however coincident with the development of antral follicles. This study thus describes the temporal and spatial regulation of early follicular development in the post-natal rabbit ovary and, for the first time, shows that the primordial to primary transition in the juvenile rabbit is a highly ordered process occurring within quantifiable parameters.
Authors:
Karla J Hutt; Eileen A McLaughlin; Michael K Holland
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-07-08
Journal Detail:
Title:  Cell and tissue research     Volume:  326     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-11-22     Completed Date:  2007-08-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  809-22     Citation Subset:  IM    
Affiliation:
Pest Animal Control Cooperative Research Centre, CSIRO Sustainable Ecosystems, G.P.O. Box 284, Canberra, ACT 2615, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Egg Proteins / analysis
Female
Granulosa Cells / cytology,  metabolism
Immunohistochemistry
Membrane Glycoproteins / analysis
Ovarian Follicle / cytology,  growth & development*,  physiology*
Ovary / growth & development*,  metabolism
Proliferating Cell Nuclear Antigen / analysis
Rabbits
Receptors, Cell Surface / analysis
Time Factors
Chemical
Reg. No./Substance:
0/Egg Proteins; 0/Membrane Glycoproteins; 0/Proliferating Cell Nuclear Antigen; 0/Receptors, Cell Surface; 0/zona pellucida glycoproteins

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