Document Detail

Primordial linkage of β2-microglobulin to the MHC.
MedLine Citation:
PMID:  21321107     Owner:  NLM     Status:  MEDLINE    
β2-Microglobulin (β2M) is believed to have arisen in a basal jawed vertebrate (gnathostome) and is the essential L chain that associates with most MHC class I molecules. It contains a distinctive molecular structure called a constant-1 Ig superfamily domain, which is shared with other adaptive immune molecules including MHC class I and class II. Despite its structural similarity to class I and class II and its conserved function, β2M is encoded outside the MHC in all examined species from bony fish to mammals, but it is assumed to have translocated from its original location within the MHC early in gnathostome evolution. We screened a nurse shark bacterial artificial chromosome library and isolated clones containing β2M genes. A gene present in the MHC of all other vertebrates (ring3) was found in the bacterial artificial chromosome clone, and the close linkage of ring3 and β2M to MHC class I and class II genes was determined by single-strand conformational polymorphism and allele-specific PCR. This study satisfies the long-held conjecture that β2M was linked to the primordial MHC (Ur MHC); furthermore, the apparent stability of the shark genome may yield other genes predicted to have had a primordial association with the MHC specifically and with immunity in general.
Yuko Ohta; Takashi Shiina; Rebecca L Lohr; Kazuyoshi Hosomichi; Toni I Pollin; Edward J Heist; Shingo Suzuki; Hidetoshi Inoko; Martin F Flajnik
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-02-14
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  186     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-03     Completed Date:  2011-05-26     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3563-71     Citation Subset:  AIM; IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB571627;  HM625830;  HM625831
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MeSH Terms
Base Sequence
Conserved Sequence / genetics,  immunology
Genes, MHC Class I / immunology*
Genes, MHC Class II / immunology*
Genetic Linkage / immunology*
Molecular Sequence Data
Sharks / genetics*,  immunology*,  metabolism
beta 2-Microglobulin / chemistry*,  genetics*
Grant Support
Reg. No./Substance:
0/beta 2-Microglobulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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