Document Detail


Priming with a very low dose of DNA complexed with cationic block copolymers followed by protein boost elicits broad and long-lasting antigen-specific humoral and cellular responses in mice.
MedLine Citation:
PMID:  19450649     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cationic block copolymers spontaneously assemble via electrostatic interactions with DNA molecules in aqueous solution giving rise to micellar structures that protect the DNA from enzymatic degradation both in vitro and in vivo. In addition, we have previously shown that they are safe, not immunogenic and greatly increased antigen-specific CTL responses following six intramuscular inoculations of a very low dose (1microg) of the vaccine DNA as compared to naked DNA. Nevertheless, they failed to elicit detectable humoral responses against the antigen. To gain further insight in the potential application of this technology, here we show that a shorter immunization protocol based on two DNA intramuscular inoculations of 1microg of DNA delivered by these copolymers and a protein boost elicits in mice broad (both humoral and cellular) and long-lasting responses and increases the antigen-specific Th1-type T cell responses and CTLs as compared to priming with naked DNA. These results indicate that cationic block copolymers represent a promising adjuvant and delivery technology for DNA vaccination strategies aimed at combating intracellular pathogens.
Authors:
Rebecca Voltan; Arianna Castaldello; Egidio Brocca-Cofano; Rita De Michele; Chiara Triulzi; Giuseppe Altavilla; Luisa Tondelli; Michele Laus; Katia Sparnacci; Eva Reali; Riccardo Gavioli; Barbara Ensoli; Antonella Caputo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-28
Journal Detail:
Title:  Vaccine     Volume:  27     ISSN:  1873-2518     ISO Abbreviation:  Vaccine     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-03     Completed Date:  2009-08-13     Revised Date:  2009-08-20    
Medline Journal Info:
Nlm Unique ID:  8406899     Medline TA:  Vaccine     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  4498-507     Citation Subset:  IM    
Affiliation:
Department of Histology, Microbiology and Medical Biotechnology, University of Padova, Padova, Italy.
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MeSH Terms
Descriptor/Qualifier:
AIDS Vaccines / administration & dosage,  immunology
Adjuvants, Immunologic / administration & dosage
Animals
Cations / immunology
Cell Proliferation
Cytokines / immunology
Epitope Mapping
Female
HIV Antibodies / blood,  immunology
HIV Antigens / immunology
Immunity, Cellular
Immunoglobulin G / blood,  immunology
Injections, Intramuscular
Mice
Mice, Inbred BALB C
Polymers / pharmacology*
Spleen / cytology,  immunology
T-Lymphocytes, Cytotoxic / immunology*
Th1 Cells / immunology
Vaccines, DNA / administration & dosage,  immunology*
tat Gene Products, Human Immunodeficiency Virus / immunology
Chemical
Reg. No./Substance:
0/AIDS Vaccines; 0/Adjuvants, Immunologic; 0/Cations; 0/Cytokines; 0/HIV Antibodies; 0/HIV Antigens; 0/Immunoglobulin G; 0/Polymers; 0/Vaccines, DNA; 0/tat Gene Products, Human Immunodeficiency Virus

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