| Priming with a very low dose of DNA complexed with cationic block copolymers followed by protein boost elicits broad and long-lasting antigen-specific humoral and cellular responses in mice. | |
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MedLine Citation:
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PMID: 19450649 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cationic block copolymers spontaneously assemble via electrostatic interactions with DNA molecules in aqueous solution giving rise to micellar structures that protect the DNA from enzymatic degradation both in vitro and in vivo. In addition, we have previously shown that they are safe, not immunogenic and greatly increased antigen-specific CTL responses following six intramuscular inoculations of a very low dose (1microg) of the vaccine DNA as compared to naked DNA. Nevertheless, they failed to elicit detectable humoral responses against the antigen. To gain further insight in the potential application of this technology, here we show that a shorter immunization protocol based on two DNA intramuscular inoculations of 1microg of DNA delivered by these copolymers and a protein boost elicits in mice broad (both humoral and cellular) and long-lasting responses and increases the antigen-specific Th1-type T cell responses and CTLs as compared to priming with naked DNA. These results indicate that cationic block copolymers represent a promising adjuvant and delivery technology for DNA vaccination strategies aimed at combating intracellular pathogens. |
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Authors:
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Rebecca Voltan; Arianna Castaldello; Egidio Brocca-Cofano; Rita De Michele; Chiara Triulzi; Giuseppe Altavilla; Luisa Tondelli; Michele Laus; Katia Sparnacci; Eva Reali; Riccardo Gavioli; Barbara Ensoli; Antonella Caputo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-28 |
Journal Detail:
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Title: Vaccine Volume: 27 ISSN: 1873-2518 ISO Abbreviation: Vaccine Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-07-03 Completed Date: 2009-08-13 Revised Date: 2009-08-20 |
Medline Journal Info:
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Nlm Unique ID: 8406899 Medline TA: Vaccine Country: Netherlands |
Other Details:
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Languages: eng Pagination: 4498-507 Citation Subset: IM |
Affiliation:
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Department of Histology, Microbiology and Medical Biotechnology, University of Padova, Padova, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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AIDS Vaccines
/
administration & dosage,
immunology Adjuvants, Immunologic / administration & dosage Animals Cations / immunology Cell Proliferation Cytokines / immunology Epitope Mapping Female HIV Antibodies / blood, immunology HIV Antigens / immunology Immunity, Cellular Immunoglobulin G / blood, immunology Injections, Intramuscular Mice Mice, Inbred BALB C Polymers / pharmacology* Spleen / cytology, immunology T-Lymphocytes, Cytotoxic / immunology* Th1 Cells / immunology Vaccines, DNA / administration & dosage, immunology* tat Gene Products, Human Immunodeficiency Virus / immunology |
| Chemical | |
Reg. No./Substance:
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0/AIDS Vaccines; 0/Adjuvants, Immunologic; 0/Cations; 0/Cytokines; 0/HIV Antibodies; 0/HIV Antigens; 0/Immunoglobulin G; 0/Polymers; 0/Vaccines, DNA; 0/tat Gene Products, Human Immunodeficiency Virus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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