Document Detail


Priming type II polyketide synthases via a type II nonribosomal peptide synthetase mechanism.
MedLine Citation:
PMID:  16448095     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Benzoic acid priming of the enterocin and actinorhodin type II polyketide synthase complexes was accomplished in vitro via an unprecedented type II nonribosomal peptide synthetase-like mechanism involving the benzoate:acyl carrier protein (ACP) ligase EncN and the ACP EncC. The transfer of the aryl acid to the ACP is ATP-dependent, yet coenzyme A-independent, as characterized with radiolabeled substrates and protein mass spectrometry. Subsequent transport of the ACP-bound aryl group to the native enterocin and the aberrant actinorhodin ketosynthase chain length factor heterodimers was further demonstrated, thereby demonstrating the potential of this biocatalyst for engineering diverse aryl-primed aromatic polyketide agents.
Authors:
Miho Izumikawa; Qian Cheng; Bradley S Moore
Related Documents :
9714775 - Characterization of triacylglycerol biosynthesis in subcellular fractions of an oleagin...
17549435 - Lipase-catalyzed regioselective synthesis of steryl (6'-o-acyl)glucosides.
2862845 - Isolation and characterization of dl-methylmalonyl-coenzyme a racemase from rat liver.
6147345 - Generation of fatty acids by an acyl esterase in the bioluminescent system of photobact...
6131935 - Release of endogenous amino acids from superior colliculus of the rabbit: in vitro stud...
18953075 - Fasting and postprandial serum bile acid concentrations in 10 healthy female red-eared ...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  128     ISSN:  0002-7863     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-01     Completed Date:  2006-04-03     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1428-9     Citation Subset:  IM    
Affiliation:
College of Pharmacy, University of Arizona, Tucson, Arizona 85721, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acyl Carrier Protein / chemistry,  metabolism
Anthraquinones / chemistry,  metabolism
Coenzyme A / chemistry,  metabolism
Naphthacenes / chemistry,  metabolism
Peptide Synthases / chemistry,  metabolism*
Polyketide Synthases / chemistry,  metabolism*
Grant Support
ID/Acronym/Agency:
AI47818/AI/NIAID NIH HHS; R01 AI047818-05/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Acyl Carrier Protein; 0/Anthraquinones; 0/Naphthacenes; 1397-77-9/actinorhodin; 146864-75-7/5838 DNI; 79956-01-7/Polyketide Synthases; 85-61-0/Coenzyme A; EC 6.3.2.-/Peptide Synthases; EC 6.3.2.-/non-ribosomal peptide synthase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A new approach to promoting sluggish Diels-Alder reactions: dihapto-coordination of the diene.
Next Document:  Ion pair driven self-assembly of a flexible bis-zwitterion in polar solution: formation of discrete ...