| Priming type II polyketide synthases via a type II nonribosomal peptide synthetase mechanism. | |
| | |
MedLine Citation:
|
PMID: 16448095 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Benzoic acid priming of the enterocin and actinorhodin type II polyketide synthase complexes was accomplished in vitro via an unprecedented type II nonribosomal peptide synthetase-like mechanism involving the benzoate:acyl carrier protein (ACP) ligase EncN and the ACP EncC. The transfer of the aryl acid to the ACP is ATP-dependent, yet coenzyme A-independent, as characterized with radiolabeled substrates and protein mass spectrometry. Subsequent transport of the ACP-bound aryl group to the native enterocin and the aberrant actinorhodin ketosynthase chain length factor heterodimers was further demonstrated, thereby demonstrating the potential of this biocatalyst for engineering diverse aryl-primed aromatic polyketide agents. |
| | |
Authors:
|
Miho Izumikawa; Qian Cheng; Bradley S Moore |
Related Documents
:
|
9714775 - Characterization of triacylglycerol biosynthesis in subcellular fractions of an oleagin... 17549435 - Lipase-catalyzed regioselective synthesis of steryl (6'-o-acyl)glucosides. 2862845 - Isolation and characterization of dl-methylmalonyl-coenzyme a racemase from rat liver. 6147345 - Generation of fatty acids by an acyl esterase in the bioluminescent system of photobact... 6131935 - Release of endogenous amino acids from superior colliculus of the rabbit: in vitro stud... 18953075 - Fasting and postprandial serum bile acid concentrations in 10 healthy female red-eared ... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Journal of the American Chemical Society Volume: 128 ISSN: 0002-7863 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2006 Feb |
Date Detail:
|
Created Date: 2006-02-01 Completed Date: 2006-04-03 Revised Date: 2013-04-12 |
Medline Journal Info:
|
Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
|
Languages: eng Pagination: 1428-9 Citation Subset: IM |
Affiliation:
|
College of Pharmacy, University of Arizona, Tucson, Arizona 85721, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acyl Carrier Protein
/
chemistry,
metabolism Anthraquinones / chemistry, metabolism Coenzyme A / chemistry, metabolism Naphthacenes / chemistry, metabolism Peptide Synthases / chemistry, metabolism* Polyketide Synthases / chemistry, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
AI47818/AI/NIAID NIH HHS; R01 AI047818-05/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Acyl Carrier Protein; 0/Anthraquinones; 0/Naphthacenes; 1397-77-9/actinorhodin; 146864-75-7/5838 DNI; 79956-01-7/Polyketide Synthases; 85-61-0/Coenzyme A; EC 6.3.2.-/Peptide Synthases; EC 6.3.2.-/non-ribosomal peptide synthase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A new approach to promoting sluggish Diels-Alder reactions: dihapto-coordination of the diene.
Next Document: Ion pair driven self-assembly of a flexible bis-zwitterion in polar solution: formation of discrete ...