Document Detail

Primary structures and biological activities of substance-P-related peptides from the brain of the dogfish, Scyliorhinus canicula.
MedLine Citation:
PMID:  7685693     Owner:  NLM     Status:  MEDLINE    
Two peptides with substance-P-like immunoreactivity were isolated in pure form from an extract of the brain of the elasmobranch fish, Scyliorhinus canicula (european common dogfish). One peptide was identical to scyliorhinin I, previously identified in dogfish intestine, and the second was the undecapeptide Lys-Pro-Arg-Pro-Gly-Gln-Phe-Phe-Gly-Leu-Met-CONH2 which is structurally similar to mammalian substance P. Scyliorhinin II or a peptide analogous to mammalian neurokinin A were not detected in the extract. Synthetic dogfish substance P ([Lys1, Arg3, Gly5]substance P) was approximately threefold more potent than mammalian substance P (Kd = 0.21 +/- 0.11 nM versus Kd = 0.74 +/- 0.17 nM; mean +/- SD; n = 6) in inhibiting the binding of 125I-labelled substance P to neurokinin (NK1) receptors in rat submandibular gland membranes. The vasodilator action of tachykinins in mammals is mediated primarily through interaction with NK1 receptors. Bolus intravenous injections of [Lys1, Arg3, Gly5]substance P (100 pmol) and scyliorhinin I (100 pmol) produced appreciable (> 4 kPa) decreases in arterial blood pressure in the rat whereas intravenous injections of up to 5 nmol of the peptides into conscious, unrestrained dogfish produced no change in arterial blood pressure, pulse amplitude or heart rate. Injections of greater amounts of the peptides (10-50 nmol) produced a slight increase (400-667 Pa) in blood pressure. The data indicate that mammalian-type NK1 tachykinin receptors are not involved in cardiovascular regulation in elasmobranch fish.
D Waugh; Y Wang; N Hazon; R J Balment; J M Conlon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  214     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1993 Jun 
Date Detail:
Created Date:  1993-07-19     Completed Date:  1993-07-19     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  469-74     Citation Subset:  IM    
Regulatory Peptide Center, Creighton University School of Medicine, Omaha, NE 68178.
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MeSH Terms
Amino Acid Sequence
Binding, Competitive
Blood Pressure / drug effects
Brain Chemistry*
Cell Membrane / metabolism
Heart Rate / drug effects
Molecular Sequence Data
Rats, Sprague-Dawley
Submandibular Gland / metabolism
Substance P / chemistry*,  metabolism,  pharmacology
Tachykinins / analysis,  chemistry,  metabolism,  pharmacology
Reg. No./Substance:
0/Tachykinins; 103425-21-4/scyliorhinin I; 33507-63-0/Substance P

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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