Document Detail


Primary structure of a thrombin-like serine protease, kangshuanmei, from the venom of Agkistrodon halys brevicaudus stejneger.
MedLine Citation:
PMID:  16908038     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The complete amino acid sequence of the thrombin-like serine protease, named kangshuanmei, isolated from the venom of a Chinese snake Agkistrodon halys brevicaudus stejneger, was determined by Edman degradation. The serine protease was composed of 236 amino acid residues and conserved the catalytic triad as His43, Asp88 and Ser182. The protease had four sites of asparagine-linked glycosylation at 81, 99, 148 and 229, and contained fucose, N-acetylglucosamin, galactose, mannose and N-acetylneuraminic acid. The amino acid sequence exhibited considerable similarities with other thrombin-like proteases isolated from the snake venoms of the Viperidae family. However, the enzymatic characteristics of kangshuanmei distinct from that of thrombin and the other protease from the venom of Viperidae family may be derived from the structural difference of the sequence in the functional regions, especially corresponding to thrombin exosite 1, 2 and hydrophobic pocket.
Authors:
Junichi Sakai; Shujuan Zhang; Hongmiao Chen; Fukiko Atsumi; Takuya Matsui; Hiroyuki Shiono; Susumu Sanada; Tadashi Okada
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Publication Detail:
Type:  Journal Article     Date:  2006-06-27
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  48     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-18     Completed Date:  2006-10-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  England    
Other Details:
Languages:  eng     Pagination:  313-22     Citation Subset:  IM    
Affiliation:
Department of Physiology, Aichi Medical University School of Medicine, Nagakute, Aichi 480-1195, Japan. j_sakai@aichi-med-u.ac.jp <j_sakai@aichi-med-u.ac.jp>
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MeSH Terms
Descriptor/Qualifier:
Agkistrodon
Amino Acid Sequence
Animals
Catalytic Domain
Crotalid Venoms / enzymology*
Glycosylation
Molecular Sequence Data
Molecular Weight
Serine Endopeptidases / chemistry*
Thrombin / chemistry*
Chemical
Reg. No./Substance:
0/Crotalid Venoms; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.5/Thrombin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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