| Primary structure and functional expression of rat and human stem cell factor DNAs. | |
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MedLine Citation:
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PMID: 2208279 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Partial cDNA and genomic clones of rat stem cell factor (SCF) have been isolated. Using probes based on the rat sequence, partial and full-length cDNA and genomic clones of human SCF have been isolated. Based on the primary structure of the 164 amino acid protein purified from BRL-3A cells, truncated forms of the rat and human proteins have been expressed in E. coli and mammalian cells and have been shown to possess biological activity. SCF is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow cultures. SCF exhibits potent synergistic activities in conjunction with colony-stimulating factors, resulting in increased colony numbers and colony size. |
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Authors:
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F H Martin; S V Suggs; K E Langley; H S Lu; J Ting; K H Okino; C F Morris; I K McNiece; F W Jacobsen; E A Mendiaz |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Cell Volume: 63 ISSN: 0092-8674 ISO Abbreviation: Cell Publication Date: 1990 Oct |
Date Detail:
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Created Date: 1990-11-09 Completed Date: 1990-11-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0413066 Medline TA: Cell Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 203-11 Citation Subset: IM |
Affiliation:
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AMGEN Inc., AMGEN Center, Thousand Oaks, California 91320. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/M59964; M59966 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Cell Division / drug effects Cloning, Molecular DNA / genetics*, isolation & purification Gene Expression Genes Genomic Library Growth Substances / pharmacology Hematopoietic Cell Growth Factors / genetics*, isolation & purification, pharmacology Humans Molecular Sequence Data Oligonucleotide Probes Plasmids Protein Conformation Rats Recombinant Proteins / isolation & purification, pharmacology Sequence Homology, Nucleic Acid |
| Chemical | |
Reg. No./Substance:
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0/Growth Substances; 0/Hematopoietic Cell Growth Factors; 0/Oligonucleotide Probes; 0/Recombinant Proteins; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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