Document Detail

Primary structure and expression of a pathogen-induced protease (PR-P69) in tomato plants: Similarity of functional domains to subtilisin-like endoproteases.
MedLine Citation:
PMID:  8692815     Owner:  NLM     Status:  MEDLINE    
A 69-kDa proteinase (P69), a member of the pathogenesis-related proteins, is induced and accumulates in tomato (Lycopersicon esculentum) plants as a consequence of pathogen attack. We have used the polymerase chain reaction to identify and clone a cDNA from tomato plants that represent the pathogenesis-related P69 proteinase. The nucleotide sequence analysis revealed that P69 is synthesized in a preproenzyme form, a 745-amino acid polypeptide with a 22-amino acid signal peptide, a 92-amino acid propolypeptide, and a 631-amino acid mature polypeptide. Within the mature region the most salient feature was the presence of domains homologous to the subtilisin serine protease family. The amino acid sequences surrounding Asp-146, His-203, and Ser-532 of P69 are closely related to the catalytic sites (catalytic triad) of the subtilisin-like proteases. Northern blot analysis revealed that the 2.4-kb P69 mRNA accumulates abundantly in leaves and stem tissues from viroid-infected plants, whereas the mRNA levels in tissues from healthy plants were undetectable. Our results indicate that P69, a secreted calcium-activated endopeptidase, is a plant pathogenesis-related subtilisin-like proteinase that may collaborate with other defensive proteins in a general mechanism of active defense against attacking pathogens.
P Tornero; V Conejero; P Vera
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  93     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1996-08-23     Completed Date:  1996-08-23     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6332-7     Citation Subset:  IM    
Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica-Consejo Superior de Investigaciones Científicas, Valencia, Spain.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Blotting, Northern
Cloning, Molecular
Lycopersicon esculentum / enzymology*,  virology
Molecular Sequence Data
RNA, Messenger / genetics
Sequence Homology, Amino Acid
Subtilisins / genetics*
Reg. No./Substance:
0/RNA, Messenger; EC 3.4.21.-/Subtilisins; EC 3.4.21.-/pathogen-induced protease, P69

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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