| Primary structure of 21 novel monoantennary and diantennary N-linked carbohydrate chains from alphaD-hemocyanin of Helix pomatia. | |
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MedLine Citation:
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PMID: 9363772 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The primary structures of 21 novel monoantennary and diantennary N-glycans of the glycoprotein alphaD-hemocyanin (alphaD-Hc) of Helix pomatia have been determined. Outer oligosaccharide fragments (antennae) were released from the glycoprotein by Smith degradation of an alphaD-Hc pronase digest. The major antenna, obtained following HPLC fractionation on Lichrosorb-NH2, was characterized using 1H-NMR spectroscopy, fast-atom-bombardment mass spectrometry, and linkage analysis, and corresponds to a pentasaccharide fragment. The intact carbohydrate chains of alphaD-Hc were released with peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase-F digestion, separated from the protein on Bio-Gel P-100, and subfractionated on Bio-Gel P-4. A portion of subfractions was reduced with sodium borodeuteride, and the non-reduced and reduced samples were further fractionated on CarboPac PA-1, Lichrosorb-NH2/Lichrosphere-NH2, and/or Lichrosphere-C18. Purified oligosaccharides and oligosaccharide-alditols were analyzed using 500/600-MHz 1H-NMR spectroscopy. In total, four novel types of antenna were identified, namely, [structures: see text] which are all attached to O-2 of alphaMan residues of the trimannosyl-N,N'-diacetylchitobiose core element, which is generally beta-1,2-xylosylated and alpha-1,6-fucosylated, Man(alpha1-6)[Man(alpha1-3)][+/-Xyl(beta1-2)]Man(beta1-4)GlcNAc(beta1-4) [+/-Fuc(alpha1-6)]GlcNAc. |
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Authors:
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J P Lommerse; J E Thomas-Oates; C Gielens; G Préaux; J P Kamerling; J F Vliegenthart |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of biochemistry / FEBS Volume: 249 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1997 Oct |
Date Detail:
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Created Date: 1997-12-05 Completed Date: 1997-12-05 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY |
Other Details:
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Languages: eng Pagination: 195-222 Citation Subset: IM |
Affiliation:
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Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amidohydrolases Animals Carbohydrate Conformation Carbohydrate Sequence Carbohydrates / chemistry Chromatography, High Pressure Liquid Helix (Snails) / chemistry* Hemocyanin / chemistry* Magnetic Resonance Spectroscopy Molecular Sequence Data Molecular Structure Oligosaccharides / chemistry, isolation & purification Oxidation-Reduction Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase Polysaccharides / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Oligosaccharides; 0/Polysaccharides; 9013-72-3/Hemocyanin; EC 3.5.-/Amidohydrolases; EC 3.5.1.52/Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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