Document Detail


Primary structural features of rosaceous S-RNases associated with gametophytic self-incompatibility.
MedLine Citation:
PMID:  9700066     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We isolated cDNA clones encoding five S-RNases (S1-, S3-, S5-, S6-, S7-RNases) from pistils of Pyrus pyrifolia (Japanese pear), a member of the Rosaceae. Their amino acid sequences were aligned with those of other rosaceous S-RNases sequenced so far. A total of 76 conserved amino acid residues were stretched throughout the sequence, but were absent from the 51-66 region which was designated the hypervariable (HV) region. The phylogenetic tree of rosaceous S-RNases showed that S-RNase polymorphism predated the divergence of Pyrus and Malus. Pairwise comparison of these S-RNases detected two highly homologous pairs, P. pyrifolia S1- and S4-RNases (90.0%) and P. pyrifolia S3- and S5-RNases (95.5%). The positions of amino acid substitutions between S1- and S4-RNases were spread over the entire region, but in the pair of S3- and S5-RNases, amino acid substitutions were found in the 21-90 region including the HV region. The substitutions in this restricted region appear to be sufficient to discriminate between S3 and S5 pollen and to trigger the self-incompatible reaction.
Authors:
T Ishimizu; T Shinkawa; F Sakiyama; S Norioka
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant molecular biology     Volume:  37     ISSN:  0167-4412     ISO Abbreviation:  Plant Mol. Biol.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-08-28     Completed Date:  1998-08-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9106343     Medline TA:  Plant Mol Biol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  931-41     Citation Subset:  IM    
Affiliation:
Division of Protein Chemistry, Institute for Protein Research, Osaka University, Suita, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB002139;  AB002140;  AB002141;  AB002142;  AB002143
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MeSH Terms
Descriptor/Qualifier:
Alleles
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Crosses, Genetic
DNA, Complementary / genetics
Evolution, Molecular
Fertility / genetics
Fruit / enzymology,  genetics*
Haploidy
Molecular Sequence Data
Ribonucleases / genetics*
Rosales / enzymology,  genetics*
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Species Specificity
Chemical
Reg. No./Substance:
0/DNA, Complementary; EC 3.1.-/Ribonucleases; EC 3.1.4.-/ribonuclease S

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