| Primary and secondary progesterone hydroxylation by the rabbit liver microsomal cytochromes P-450 system. | |
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MedLine Citation:
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PMID: 3695518 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Progesterone metabolism by the rabbit liver microsomal cytochromes P-450 system has been investigated with reverse phase (C18) high performance liquid chromatography. Time-course studies indicated that 6 beta-OHDOC accumulated as a secondary metabolite produced by the sequential 6 beta- and 21-hydroxylation of progesterone. When 21-hydroxylation occurred first, DOC accumulated and 6-hydroxylation was reduced. 16-OHP was resistant to secondary metabolism at both the 6- and 21-positions. The variable rates of 21-hydroxylase activity between different rabbits was further influenced by the nature of C-6-function in the order of 6-oxo greater than 6 beta-OH greater than 6 beta-OH greater than 6 alpha-OH greater than 6-H greater than 6 beta-acetoxy. These results indicate the potential interaction of the microsomal cytochromes P-450 and/or products in the sequential hydroxylation of a single steroid substrate at multiple sites. |
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Authors:
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I R Senciall |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of steroid biochemistry Volume: 28 ISSN: 0022-4731 ISO Abbreviation: J. Steroid Biochem. Publication Date: 1987 Dec |
Date Detail:
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Created Date: 1988-02-20 Completed Date: 1988-02-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0260125 Medline TA: J Steroid Biochem Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 711-6 Citation Subset: IM |
Affiliation:
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Medical School, Memorial University of Newfoundland St John's, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, High Pressure Liquid Cytochrome P-450 Enzyme System / physiology* Female Hydroxylation Male Microsomes, Liver / metabolism* Progesterone / metabolism* Rabbits Structure-Activity Relationship |
| Chemical | |
Reg. No./Substance:
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57-83-0/Progesterone; 9035-51-2/Cytochrome P-450 Enzyme System |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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