Document Detail

Primary rat Sertoli and interstitial cells exhibit a differential response to cadmium.
MedLine Citation:
PMID:  2334868     Owner:  NLM     Status:  MEDLINE    
Two cell types central to the support of spermatogenesis, the Sertoli cell and the interstitial (Leydig) cell, were isolated from the same cohort of young male rats and challenged with cadmium chloride to compare their susceptibility to the metal. Both cell types were cultured under similar conditions, and similar biochemical endpoints were chosen to minimize experimental variability. These endpoints include the uptake of 109Cd, reduction of the vital tetrazolium dye MTT, incorporation of 3H-leucine, change in heat-stable cadmium binding capacity, and production of lactate. Using these parameters, it was observed that the Sertoli cell cultures were adversely affected in a dose-and time-dependent manner, while the interstitial cell cultures, treated with identical concentrations of CdCl2, were less affected. The 72-hr LC50's for Sertoli cells and interstitial cells were 4.1 and 19.6 microM CdCl2, respectively. Thus, different cell populations within the same tissue may differ markedly in susceptibility to a toxicant. These in vitro data suggest that the Sertoli cell, in relation to the interstitium, is particularly sensitive to cadmium. Because the Sertoli cell provides functional support for the seminiferous epithelium, the differential sensitivity of this cell type may, in part, explain cadmium-induced testicular dysfunction, particularly at doses that leave the vascular epithelium intact.
S R Clough; M J Welsh; A H Payne; C D Brown; M J Brabec
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell biology and toxicology     Volume:  6     ISSN:  0742-2091     ISO Abbreviation:  Cell Biol. Toxicol.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-06-14     Completed Date:  1990-06-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8506639     Medline TA:  Cell Biol Toxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  63-79     Citation Subset:  IM    
Eastern Michigan University, Department of Chemistry, Ypsilanti 48197.
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MeSH Terms
Cadmium / metabolism,  pharmacokinetics,  pharmacology*
Cells, Cultured
Fertility / drug effects
Lactates / biosynthesis,  metabolism
Leucine / metabolism
Leydig Cells / drug effects*,  metabolism,  physiology
Protein Biosynthesis
Proteins / metabolism
Rats, Inbred Strains
Sertoli Cells / drug effects*,  metabolism,  physiology
Tetrazolium Salts / metabolism
Tritium / diagnostic use
Grant Support
Reg. No./Substance:
0/Lactates; 0/Proteins; 0/Tetrazolium Salts; 10028-17-8/Tritium; 61-90-5/Leucine; 7440-43-9/Cadmium

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