| Primary rat Sertoli and interstitial cells exhibit a differential response to cadmium. | |
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MedLine Citation:
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PMID: 2334868 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two cell types central to the support of spermatogenesis, the Sertoli cell and the interstitial (Leydig) cell, were isolated from the same cohort of young male rats and challenged with cadmium chloride to compare their susceptibility to the metal. Both cell types were cultured under similar conditions, and similar biochemical endpoints were chosen to minimize experimental variability. These endpoints include the uptake of 109Cd, reduction of the vital tetrazolium dye MTT, incorporation of 3H-leucine, change in heat-stable cadmium binding capacity, and production of lactate. Using these parameters, it was observed that the Sertoli cell cultures were adversely affected in a dose-and time-dependent manner, while the interstitial cell cultures, treated with identical concentrations of CdCl2, were less affected. The 72-hr LC50's for Sertoli cells and interstitial cells were 4.1 and 19.6 microM CdCl2, respectively. Thus, different cell populations within the same tissue may differ markedly in susceptibility to a toxicant. These in vitro data suggest that the Sertoli cell, in relation to the interstitium, is particularly sensitive to cadmium. Because the Sertoli cell provides functional support for the seminiferous epithelium, the differential sensitivity of this cell type may, in part, explain cadmium-induced testicular dysfunction, particularly at doses that leave the vascular epithelium intact. |
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Authors:
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S R Clough; M J Welsh; A H Payne; C D Brown; M J Brabec |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cell biology and toxicology Volume: 6 ISSN: 0742-2091 ISO Abbreviation: Cell Biol. Toxicol. Publication Date: 1990 Jan |
Date Detail:
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Created Date: 1990-06-14 Completed Date: 1990-06-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8506639 Medline TA: Cell Biol Toxicol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 63-79 Citation Subset: IM |
Affiliation:
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Eastern Michigan University, Department of Chemistry, Ypsilanti 48197. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cadmium / metabolism, pharmacokinetics, pharmacology* Cells, Cultured Fertility / drug effects Lactates / biosynthesis, metabolism Leucine / metabolism Leydig Cells / drug effects*, metabolism, physiology Male Oxidation-Reduction Protein Biosynthesis Proteins / metabolism Rats Rats, Inbred Strains Sertoli Cells / drug effects*, metabolism, physiology Tetrazolium Salts / metabolism Tritium / diagnostic use |
| Grant Support | |
ID/Acronym/Agency:
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ESO4141/ES/NIEHS NIH HHS; HD-08358/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Lactates; 0/Proteins; 0/Tetrazolium Salts; 10028-17-8/Tritium; 61-90-5/Leucine; 7440-43-9/Cadmium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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