Document Detail


Primary fatty acid amide metabolism: conversion of fatty acids and an ethanolamine in N18TG2 and SCP cells.
MedLine Citation:
PMID:  22095832     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Primary fatty acid amides (PFAM) are important signaling molecules in the mammalian nervous system, binding to many drug receptors and demonstrating control over sleep, locomotion, angiogenesis, and many other processes. Oleamide is the best-studied of the primary fatty acid amides, whereas the other known PFAMs are significantly less studied. Herein, quantitative assays were used to examine the endogenous amounts of a panel of PFAMs, as well as the amounts produced after incubation of mouse neuroblastoma N(18)TG(2) and sheep choroid plexus (SCP) cells with the corresponding fatty acids or N-tridecanoylethanolamine. Although five endogenous primary amides were discovered in the N(18)TG(2) and SCP cells, a different pattern of relative amounts were found between the two cell lines. Higher amounts of primary amides were found in SCP cells, and the conversion of N-tridecanoylethanolamine to tridecanamide was observed in the two cell lines. The data reported here show that the N(18)TG(2) and SCP cells are excellent model systems for the study of PFAM metabolism. Furthermore, the data support a role for the N-acylethanolamines as precursors for the PFAMs and provide valuable new kinetic results useful in modeling the metabolic flux through the pathways for PFAM biosynthesis and degradation.
Authors:
Emma K Farrell; Yuden Chen; Muna Barazanji; Kristen A Jeffries; Felipe Cameroamortegui; David J Merkler
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-11-16
Journal Detail:
Title:  Journal of lipid research     Volume:  53     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-10     Completed Date:  2012-08-27     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  247-56     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of South Florida, Tampa, FL 33620-5250, USA.
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MeSH Terms
Descriptor/Qualifier:
Amides / metabolism*
Animals
Cells, Cultured
Choroid Plexus / cytology,  metabolism
Ethanolamine / metabolism*
Ethanolamines / metabolism
Fatty Acids / metabolism*
Fatty Acids, Monounsaturated / metabolism
Linoleic Acids / metabolism
Mice
Neuroblastoma / metabolism
Oleic Acids / metabolism
Palmitic Acids / metabolism
Sheep / metabolism
Sheep, Domestic
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
R15-GM-059050/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Amides; 0/Ethanolamines; 0/Fatty Acids; 0/Fatty Acids, Monounsaturated; 0/Linoleic Acids; 0/Oleic Acids; 0/Palmitic Acids; 141-43-5/Ethanolamine; 5340S2UXSP/linoleamide; 629-54-9/hexadecanamide; 7L25QK8BWO/oleylamide
Comments/Corrections

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