Document Detail


Primary cultures of nervous system cells from the larva of the ascidian Ciona intestinalis.
MedLine Citation:
PMID:  17669506     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ascidian Ciona intestinalis is a useful model for the study of nervous system development and function. The larva of this animal represents a 'primitive' vertebrate form that contains only about 100 neurons in the CNS. Although embryos can be easily subjected to genetic manipulation, the nervous system cells are not easily accessible for neurophysiological study at the larval stage. To remedy this problem, we have developed a method to obtain primary cell cultures from the larval stage of Ciona. Light microscopy and electrophysiology discriminate several types of cells including neurons and photoreceptors. The results show that in Ciona primary cultures different types of neurons as well as neurite sprouting and synapse formation can be visualised. Ciona primary cell cultures will be very useful to study the biochemical, molecular and biophysical properties of individual cells in the larval nervous system of C. intestinalis.
Authors:
Laura Zanetti; Filomena Ristoratore; Maria Francone; Stefania Piscopo; Euan R Brown
Related Documents :
9530996 - Heterogeneous patterns of oligodendroglial differentiation in the forebrain of the opos...
2177046 - Effect of acyclovir on herpes simplex virus infected neuronal, glial, and neurohybridom...
10328946 - Glial cell lineages in the rat cerebral cortex.
16288476 - Calbindin-positive neurons reveal a sexual dimorphism within the songbird analogue of t...
12684446 - Electrophysiological characterization of voltage-gated currents in defined taste cell t...
6656796 - Hypersensitivity to cell killing and mutation induction by chemical carcinogens in an e...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-21
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  165     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-13     Completed Date:  2007-11-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  191-7     Citation Subset:  IM    
Affiliation:
Biochemistry and Molecular Biology Laboratory, Stazione Zoologica Anton Dohrn, Villa Comunale, I-80121 Naples, Italy. lzanetti@szn.it
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Action Potentials / physiology
Animals
Cell Culture Techniques / methods
Cell Differentiation / physiology
Cell Survival / physiology
Cells, Cultured
Ciona intestinalis / cytology*,  growth & development*
Growth Cones / physiology,  ultrastructure
Larva / cytology,  growth & development
Nervous System / cytology*,  growth & development*
Neurites / physiology,  ultrastructure
Neurons / cytology,  physiology
Patch-Clamp Techniques
Photoreceptor Cells, Invertebrate / cytology,  growth & development
Synaptic Transmission / physiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A method of extracellular recording of neuronal activity in swimming mice.
Next Document:  Automatic spike detection based on adaptive template matching for extracellular neural recordings.