Document Detail


Primary culture of embryonic rat olfactory receptor neurons.
MedLine Citation:
PMID:  23150136     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Embryonic cells are very robust in surviving dissection and culturing protocols and easily adapt to their in vitro environment. Despite these advantages, research in the olfactory field on cultured embryonic olfactory neurons is sparse. In this study, two primary rat olfactory explant cultures of different embryonic d (E17 and E20) were established, comprising epithelium and bulb. The functionality of these neurons was tested by measuring intracellular calcium responses to cAMP-inducing agents forskolin (FSK) and 3-isobutyl-1-methylxanthine (IBMX) with fluorescence microscopy. For E17, the responsive cell fraction increased over time, from an initial 3% at the 1 d in vitro (DIV) to a maximum of 19% at 11 DIV. The response of E20 neurons fluctuated over time around a more or less stable 13%. A logistic regression analysis indicated a significant difference between both embryonic d in the response to FSK + IBMX. In addition, of these functional neurons, 23.3% of E17 and 54.3% of E20 cultures were responsive to the odorant isoamyl acetate.
Authors:
Evelien Micholt; Danny Jans; Geert Callewaert; Carmen Bartic; Jeroen Lammertyn; Bart Nicolai
Related Documents :
18427926 - Co-culture of arbuscular mycorrhiza-like fungi (piriformospora indica and sebacina verm...
23937176 - Reversing one's fortune by pushing away bad luck.
23856736 - Optimization of protease production by the fungus monacrosporium thaumasium and its act...
17512026 - Metabolic flux analysis in plants using dynamic labeling technique: application to tryp...
21538976 - Enhancing hypericin production of hypericum perforatum cell suspension culture by ozone...
12697286 - Different characteristics of rat retinal progenitor cells from different culture periods.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-13
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  -     ISSN:  1543-706X     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Biosystems-MeBioS (Mechatronics, Biostatistics and Sensors), University of Leuven, Willem de Croylaan 42, 3001, Leuven, Belgium.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Introduction of operating room checklists as a part of clinical risk management : Are there hard fac...
Next Document:  Lhx8 promote differentiation of hippocampal neural stem/progenitor cells into cholinergic neurons in...