Document Detail

Primary culture and characteristic morphologies of neurons from the cerebral ganglion of the mud crab, Scylla paramamosain.
MedLine Citation:
PMID:  20585894     Owner:  NLM     Status:  MEDLINE    
Crustacean neurons, obtained from the cerebral ganglion of the mud crab Scylla paramamosain, were successfully cultured in vitro. They maintained typical morphological characteristics and showed better outgrowth in modified Medium 199 (M199) medium than that in Liebowitz's L-15 medium. Fetal bovine serum (FBS), muscle extracts, and hemolymph of the mud crab S. paramamosain were added as supplements. Only 20% FBS could promote neuron outgrowth, while muscle extracts and hemolymph of S. paramamosain did not improve neuron outgrowth. For cell dissociation, both collagenase type I and trypsin worked well as determined by initial cell viability and following cell outgrowth potential. More than six kinds of cells with different morphological characteristics were identified in the neuron outgrowth. They were "small cells", "veilers", "branchers", "multipolar cells", "super-large cell", and "bipolar cells". Among all of the cells, bipolar cells were identified for the first time in crustacean neurons culture and they could live longer than other cells. The neurons could grow for more than a week before retraction and eventual degradation.
Yan Xu; Haihui Ye; Jun Ma; Huiyang Huang; Guizhong Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-29
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  46     ISSN:  1543-706X     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-30     Completed Date:  2010-12-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  708-17     Citation Subset:  IM    
Department of Oceanography, Xiamen University, Xiamen, 361005, China.
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MeSH Terms
Brachyura / cytology*
Cell Culture Techniques
Cell Survival
Ganglia, Invertebrate / cytology*
Hemolymph / physiology
Neurons / cytology*

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