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Primary pulmonary biphasic synovial sarcoma confirmed by molecular detection of a SYT-SSX2 fusion gene: report of 1 case.
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PMID:  20830232     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We experienced a case of primary pulmonary biphasic synovial sarcoma, which was confirmed by immunohistochemistry and molecular testing of SYT-SSX2 fusion transcripts. The patient was a 61-year-old man who presented with a well-defined mass in the left upper lung field on chest radiography. Left upper lobectomy with lymph node dissection was performed. Histological and immunophenotypic features were consistent with biphasic synovial sarcoma. Reverse transcriptase polymerase chain reaction, performed using RNA extracted from frozen tumor samples for the detection of SYT-SSX fusion gene, amplified a single 331-bp fragment that was characteristic of the SYT-SSX2 fusion transcripts. We report a case of primary pulmonary biphasic synovial sarcoma, which was confirmed by SYT-SSX2 fusion transcripts, and present a brief review of the literature on Korean cases.
Authors:
Jae Seok Park; Bo Ram Min; Soon Hyo Park; Kun Young Kwon; Dong-Yoon Keum; Won-Il Choi
Publication Detail:
Type:  Case Reports; Journal Article; Review     Date:  2010-08-31
Journal Detail:
Title:  The Korean journal of internal medicine     Volume:  25     ISSN:  1226-3303     ISO Abbreviation:  Korean J. Intern. Med.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-10     Completed Date:  2010-11-05     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  8712418     Medline TA:  Korean J Intern Med     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  331-6     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, Dongsan Medical Center, Keimyung University School of Medicine, Daegu, Korea.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
DNA Primers / genetics
Humans
Korea
Lung Neoplasms / diagnosis,  genetics*
Male
Middle Aged
Oncogene Proteins, Fusion / genetics*
Oncogenes
Sarcoma, Synovial / diagnosis,  genetics*
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Oncogene Proteins, Fusion; 0/SYT-SSX fusion protein
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Journal Information
Journal ID (nlm-ta): Korean J Intern Med
Journal ID (publisher-id): KJIM
ISSN: 1226-3303
ISSN: 2005-6648
Publisher: The Korean Association of Internal Medicine
Article Information
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Copyright © 2010 The Korean Association of Internal Medicine
Received Day: 28 Month: 12 Year: 2007
Revision Received Day: 12 Month: 2 Year: 2008
Accepted Day: 13 Month: 5 Year: 2008
Print publication date: Month: 9 Year: 2010
Electronic publication date: Day: 31 Month: 8 Year: 2010
Volume: 25 Issue: 3
First Page: 331 Last Page: 336
ID: 2932948
PubMed Id: 20830232
DOI: 10.3904/kjim.2010.25.3.331

Primary Pulmonary Biphasic Synovial Sarcoma Confirmed by Molecular Detection of a SYT-SSX2 Fusion Gene: Report of 1 Case
Jae Seok Park1
Bo Ram Min1
Soon Hyo Park1
Kun Young Kwon2
Dong-Yoon Keum3
Won-Il Choi1
1Department of Internal Medicine, Dongsan Medical Center, Keimyung University School of Medicine, Daegu, Korea.
2Department of Pathology, Dongsan Medical Center, Keimyung University School of Medicine, Daegu, Korea.
3Department of Thoracic Surgery, Dongsan Medical Center, Keimyung University School of Medicine, Daegu, Korea.
Correspondence: Correspondence to Won Il Choi, M.D. Department of Internal Medicine, Dongsan Medical Center, Keimyung University School of Medicine, Dongsan-dong, Jung-gu, Daegu 700-712, Korea. Tel: 82-53-250-7572, Fax: 82-53-250-7434, wichoi@dsmc.or.kr

INTRODUCTION

Pulmonary sarcoma usually occurs through metastasis from other locations such as the extremities, and primary pulmonary sarcoma accounts for < 0.5% of all pulmonary malignancies [1]. Cytogenetic studies have detected the chromosomal translocation t (x;18) (p11;q11) in synovial sarcoma and have determined that the translocation fuses the SYT gene from chromosome 18 with the SSX1 and SSX2 genes on chromosome X to form SYT-SSX1 or SYT-SSX2, which is thought to function as an aberrant transcriptional regulator [1-3]. The fusion genes detected by reverse transcriptase polymerase chain reaction (RT-PCR) have been used to make a definitive diagnosis of synovial sarcoma [3], and it has been reported that they are related to the histological characteristics and prognosis of this tumor. It has also been demonstrated that patients with the SYT-SSX2 fusion transcripts have a better prognosis than those with SYT-SSXI fusion transcripts [2].

Only a few reports have examined primary pulmonary synovial sarcoma in Korea. Here, we report a case of primary pulmonary biphasic synovial sarcoma that was confirmed by detection of an SYT-SSX2 fusion gene, and present a brief review of the literature concerning this form of tumor in Korean patients.


CASE REPORT

A 61-year-old man presented with an incidentally found mass in the lung, which was detected by a health-screening program. He had a smoking history of 30 pack-years. On admission, his vital signs were as follows: blood pressure, 120/80 mmHg; pulse rate, 75/min; respiration rate, 20/min; and body temperature, 36.5℃. He was not chronically ill, and he had clear consciousness. Chest auscultation revealed no abnormal heart and lung sounds. No enlarged lymph nodes were found in the head, neck, or extremities. Simple chest X-ray revealed a well-defined round mass in the left upper lung field (Fig. 1A). Computed tomography (CT) of the chest showed a 3 × 3-cm, well-defined, lobulated round mass in the anterior segment of the left upper lobe, and on contrast images, the mass showed heterogeneous enhancement with ≥ 20 HU difference in enhancement (Fig. 1B). Positron emission tomography (PET)-CT showed a pulmonary mass at the same site with an increased F18-fluorodeoxyglucose (FDG) standardized uptake value (SUV) of 8.1. No increase in the FDG uptake rate in the lymph nodes was detected at the mediastinum and both pulmonary hila (Fig. 2). Radioisotope bone scans revealed no abnormal findings. Peripheral blood tests showed white blood cells, 7,770/mm3; hemoglobin, 13.4 g/dL; and platelets 258,000/mm3. No abnormal findings were observed in routine chemistry tests, serum electrolytes, blood coagulation tests, and urinalysis.

Light microscopic examination of the specimens obtained through percutaneous lung biopsy revealed a spindle-cell-type neoplastic hyperplasia and localized areas of epithelial cells around the spindle cells. Immunohistochemistry displayed positive reactivity for Bcl-2, CD99, and β-catenin, with a strong possibility of a solitary fibrous tumor. However, a definite diagnosis could not be made because the tumor was negative for CD34, for which most solitary fibrous tumors are positive.

Left upper lobe resection with lymph node dissection was performed, and a 3.8 × 2.8 × 25-cm, well-defined, whitish-yellow, smooth-surfaced oval mass was found. Light microscopic examination of the surgical specimen showed spindle cells and focal epithelial cells with mitotic activity of 10/10 HPFs (Fig. 3). By immunohistochemistry, the epithelial cells were positive for epithelial membrane antigen (EMA), whereas the spindle cells were negative. In addition, the tumor was positive for S-100, CD99, and Bcl-2, but negative for CD34, desmin, cytokeratin pan (CKpan), thyroid transcription factor 1 (TTF-1), surfactant protein A (SP-A), leading to a diagnosis of primary pulmonary biphasic synovial sarcoma (Fig. 4).

RT-PCR was performed according to the method of Kawai et al. [2]. Total RNA was isolated from the frozen sections obtained from the tumor tissue using the acid/guanidinum/phenol/chloroform method, and 1 µg RNA was transcribed using Superscript II Reverse Transcriptase (GIBCO, Gaithersburg, MD, USA) and random hexamers. The cDNA was amplified using the forward primer (5'CAACAGCAAGATGCATACCA3') and reverse primer (5'CACTTGCTATGCACCTGATG3' for SSX; 5'GGTGCAGTTGTTTCCCATCG3' for SSX1; or 5'GGCACAGCTCTTTCCCATCA3' for SSX2). RT-PCR was carried out under the following conditions: 35 amplification cycles, each consisting of denaturation at 95℃ for 1 minute, annealing at 58℃ or 60℃ for 1 minute, and extension at 72℃ for 1 minute, followed by 10 minutes of an additional extension step at 72℃. The PCR products were then electrophoresed on a 1.5% agarose gel, stained with ethidium bromide, and subsequently identified as an SYT-SSX (585 bp) or SYT-SSX2 (331 bp) gene (Fig. 5). Chest radiographs taken at 16 months after surgery showed pleural effusion on the left side. Chest CT revealed a left pleura-based mass, enlarged mediastinal lymph nodes, and suspected malignant hydrothorax. PET-CT scans showed a left pleural mass with an increased F18-FDG SUV of 6.5. A percutaneous lung biopsy was performed to make a definitive diagnosis of the left pleura-based mass. Light microscopy and immunohistochemistry confirmed the recurrence of synovial sarcoma. The patient was scheduled for total resection of the tumor along with adjacent chemoradiotherapy.


DISCUSSION

Synovial sarcoma can be histopathologically divided into biphasic, monosphasic fibrous, monophasic epithelial, and poorly differentiated types. In cases of the biphasic type, both epithelial and spindle cells are present; therefore, the diagnosis is relatively easy to make. However, in cases of the monophasic type, which has homogeneous patterns of spindle cells, immunohistochemical staining is needed to distinguish this tumor from other spindle-cell-type tumors such as fibrosarcoma, hemangiopericytoma, leiomyosarcoma, and spindle cell carcinoma [1]. Coindre et al. [3] have suggested that the most reliable findings are positivity for EMA or cytokeratins AE1/AE3 and negativity for CD34. However, Begueret et al. [4] have reported that this tumor can be positive for the S-100 protein, α-smooth muscle actin, c-kit, CD34, and calretinin. Based on these results, it is conceivable that immunohistochemistry is not the most accurate test for synovial sarcoma. In our case, the tumor mass was negative for CD34, which distinguished this tumor from fibrosar-coma. EMA-negative spindle cells were observed, in contrast to EMA-positive epithelial cells. Based on these findings, our case was diagnosed as a biphasic synovial sarcoma.

Recently, through cytogenetic studies, the translocation of specific genes has been identified in various synovial-sarcoma-like forms of sarcoma. The chromosomal translocation t (x;18) (p11;q11) has been found in 95% of synovial sarcomas in which the SYT gene located on the long arm of chromosome 18 is fused with the SSX1 or SSX2 gene located on the short arm of chromosome X, forming a new SYT-SSX1 or SYT-SSX2 fusion gene. The SYT-SSX fusion gene induces the development of sarcoma. Although the biological role of the fusion gene has yet to be identified, it has been reported that the SYT protein functions as a transcriptional regulator, and the SSX protein functions as a complement for the transcriptional inhibitor [1]. The SYT-SSX1 fusion gene has been found in the majority of biphasic synovial sarcomas, whereas the SYT-SSX2 fusion gene has been detected in the majority of monophasic synovial sarcomas [2]. Nevertheless, Ladanyi et al. [5] have reported that histological classification does not always coincide with the fusion-gene classification. In our case of biphasic synovial sarcoma, an SYT-SSX2 gene was detected. The detection of fusion genes has been reported to have a sensitivity of 96% and a specificity of 100% for the diagnosis of synovial sarcoma [6], which has made this technique a widely used and important method for establishing a definite diagnosis of this tumor.

A total of six cases of primary pulmonary synovial sarcoma have been reported in Korea (Table 1) [7-12]. Our case is the first that confirmed the presence of an SYT-SSX fusion gene. Previous cases in Korea have occurred in patients aged 28 to 61 years (mean, 45.7 years), and four occurred in women. No specific symptoms were found in two cases. The tumor size was 3.5 to 10 cm (mean, 5.6 cm). The tumor developed in the left lung in five cases. As for the histological classification, five were biphasic type, one was monophasic type, and another was poorly differentiated type. The epithelial cells were positive for EMA or cytokeratin and negative for CD34. At the initial diagnosis, pneumonectomy was performed in all cases, except a case with accompanying pleural invasion and malignant pleural effusion. According to the report by Begueret et al. [4], many cases of synovial sarcoma are mistakenly diagnosed as spindle-cell tumors such as fibrosarcoma or hemangiopericytoma because of the absence of methods that give a definite diagnosis of synovial sarcoma. Recently, cases of synovial sarcoma have increased due to the aforementioned diagnostic tool. The detection of the fusion gene will assist in making a definite diagnosis of synovial sarcoma.

Several reports have examined the relationship between the SYT-SSX fusion gene and the prognosis of synovial sarcoma. Although some investigators have indicated that patients with SYT-SSX1 fusion genes have a poorer prognosis than do those with SYT-SSX2 fusion genes, Guillou et al. [6] have reported contradictory results. Further studies with a larger number of cases are needed to verify the relationship between the patterns of fusion genes and the prognosis of synovial sarcoma.

We have reported a case that was definitively diagnosed as primary pulmonary biphasic sarcoma based on the detection of an SYT-SSX2 fusion gene.


Notes

No potential conflict of interest relevant to this article was reported.

References
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Article Categories:
  • Case Report

Keywords: Sarcoma, synovial, Lung neoplasms, Gene fusion.

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