Document Detail


Preventive effect of recombinant human lactoferrin in a rabbit preterm delivery model.
MedLine Citation:
PMID:  15846177     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Lactoferrin, an iron-binding glycoprotein found in cervical mucus and amniotic fluid, plays a defensive role against mucosal infections. This study examined the effect of recombinant human lactoferrin on preterm delivery in a rabbit model. STUDY DESIGN: Anesthetized rabbits were randomly assigned to receive either inoculation with Escherichia coli or saline solution and to receive treatment with or without recombinant human lactoferrin inserted into the cervix 2 hours before bacterial inoculation (condition A: saline + saline; condition B: E coli + saline; condition C: E coli + recombinant human lactoferrin). E coli , saline solution, and recombinant human lactoferrin were inserted into the cervix using a hysteroscope and a sterile polyethylene cannula. Fetus survival rate and days to delivery after inoculation were monitored and tumor necrosis factor-alpha concentrations were measured in maternal serum and amniotic fluid. RESULTS: Fetus survival for conditions A, B, and C were 95.7%, 0%, and 32.6%, respectively, whereas pregnancy continuation was 7.00 +/- 0 days, 3.25 +/- 0.43 days, and 4.85 +/- 1.77 days, respectively. CONCLUSION: Cervical recombinant human lactoferrin administration increased fetal survival and extended pregnancy. Lactoferrin has an anti-inflammatory action as well as an antibacterial action, suggesting that recombinant human lactoferrin has the potential to prevent preterm delivery originating from cervical infection in the clinical setting.
Authors:
Akitoshi Hasegawa; Katsufumi Otsuki; Yasushi Sasaki; Maki Sawada; Kaori Mitsukawa; Hiroshi Chiba; Masaaki Nagatsuka; Takashi Okai; Atsuko Kato
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  192     ISSN:  0002-9378     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-22     Completed Date:  2005-05-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1038-43     Citation Subset:  AIM; IM    
Affiliation:
Department of Obstetrics and Gynecology, Showa University, School of Medicine, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Disease Models, Animal
Escherichia coli Infections / drug therapy*
Female
Fetal Death
Fetal Membranes, Premature Rupture / prevention & control
Humans
Immunohistochemistry
Lactoferrin / pharmacology*
Obstetric Labor, Premature / prevention & control*
Placenta / pathology
Pregnancy
Pregnancy, Animal*
Primary Prevention / methods*
Rabbits
Random Allocation
Recombinant Proteins
Reference Values
Sensitivity and Specificity
Tumor Necrosis Factor-alpha / metabolism
Chemical
Reg. No./Substance:
0/Lactoferrin; 0/Recombinant Proteins; 0/Tumor Necrosis Factor-alpha

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