| Prevention of transfusion-induced sensitization to minor histocompatibility antigens on DLA-identical canine marrow grafts by gamma irradiation of marrow donor blood. | |
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MedLine Citation:
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PMID: 1836286 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dogs given total-body irradiation and marrow transplants from DLA-identical littermates exhibit prompt and sustained hematopoietic engraftment. However, animals given three preceding blood transfusions from the marrow donor before transplant become sensitized and reject the marrow graft. Rejection is due to exposure to polymorphic minor non-DLA histocompatibility antigens expressed on blood mononuclear cells. We sought to determine whether heat treatment would prevent blood from sensitizing recipients in this model since heating blood to 45 degrees C for 45 min abrogates the ability of blood mononuclear cells to stimulate in mixed lymphocyte culture. Three of 4 evaluable dogs given heat-treated blood before transplant rejected their marrow grafts. To prevent possible reexpression/reacquisition of mononuclear cell functional activity in vivo after transfusion, subsequent dogs were given heated blood that was additionally exposed to 2000 cGy gamma irradiation. Eight of 10 evaluable dogs given blood treated in this fashion engrafted. Unexpectedly, 9 out of 10 evaluable dogs transfused with blood treated only with gamma irradiation also engrafted. These results demonstrate that treatment of blood with gamma irradiation alone or in combination with heat prevents transfusion-induced sensitization to minor histocompatibility antigens. Results from this canine model suggest that blood products be gamma irradiated before transfusion in patients who are transplant candidates in order to prevent sensitization to minor histocompatibility antigens and reduce the risk of marrow graft rejection. |
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Authors:
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M A Bean; R Storb; T Graham; R Raff; G E Sale; F Schuening; F R Appelbaum |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Transplantation Volume: 52 ISSN: 0041-1337 ISO Abbreviation: Transplantation Publication Date: 1991 Dec |
Date Detail:
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Created Date: 1992-01-23 Completed Date: 1992-01-23 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0132144 Medline TA: Transplantation Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 956-60 Citation Subset: IM |
Affiliation:
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Pacific Northwest Research Foundation, Seattle, WA 98122. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood / radiation effects Blood Transfusion* Bone Marrow / radiation effects* Bone Marrow Transplantation / immunology* Dogs Erythrocyte Aging / radiation effects Gamma Rays* Histocompatibility Antigens / analysis* Hot Temperature Leukocytes, Mononuclear / immunology Lymphocyte Culture Test, Mixed |
| Grant Support | |
ID/Acronym/Agency:
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CA 18105/CA/NCI NIH HHS; CA 18221/CA/NCI NIH HHS; CA 31787/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Histocompatibility Antigens |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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