Document Detail


Prevention of thrombocytopenia by thrombopoietin in myelosuppressed rhesus monkeys accompanied by prominent erythropoietic stimulation and iron depletion.
MedLine Citation:
PMID:  9207438     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effectiveness of thrombopoietin (TPO) in alleviating thrombocytopenia was evaluated in a placebo-controlled study involving rhesus monkeys exposed to 5 Gy total-body irradiation (TBI) (300-kV x-rays) to result in 3 weeks of pancytopenia. Supraoptimal treatment with human recombinant TPO (10 microg/kg/d subcutaneously, days 1 to 21 after TBI) was highly effective in preventing thrombocytopenia, with nadirs for thrombocytes, on average, far higher than 100 x 10(9)/L, a greatly accelerated recovery to normal values, and no need for thrombocyte transfusions. TPO appeared to act selectively in that neutrophil regeneration was not influenced but red blood cell lineage recovery was prominently stimulated, with reticulocyte regeneration being initiated 10 days earlier than in placebo-treated animals. The reticulocytosis was followed by a normoblastosis that occurred earlier and was more pronounced than in placebo-treated monkeys. The effect of TPO on the red blood cell lineage was also reflected in a less profound nadir for hemoglobin (Hb) and hematocrit values than in placebo controls. However, this effect was not followed by a rapid recovery to normal values, due to development of a microcytic hypochromic anemia. Iron depletion was demonstrated by measurements of total serum iron and total iron-binding capacity (TIBC) and could be prevented by prophylactic intramuscular (IM) iron before TBI or corrected by IM iron after TPO treatment. Rechallenging with TPO in week 8 after TBI demonstrated a homogenous thrombocyte response similar in magnitude to the initial response, but a greatly diminished reticulocyte response. This demonstrated that the erythropoietic response to TPO administration depends on the hemopoietic state of the animal and may reflect multiple TPO target cells. It is postulated that the extremely rapid erythropoiesis due to TPO treatment in the initial regeneration phase following myelosuppression results in iron depletion by a mechanism similar to that seen following erythropoietin treatment in patients with end-stage renal failure. It is concluded that protracted TPO therapy to counteract thrombocytopenic states may result in iron depletion and that the iron status should be monitored before, during, and after TPO treatment.
Authors:
K J Neelis; L Qingliang; G R Thomas; B L Cohen; D L Eaton; G Wagemaker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Blood     Volume:  90     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-07-22     Completed Date:  1997-07-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  58-63     Citation Subset:  AIM; IM    
Affiliation:
Institute of Hematology, Erasmus University Rotterdam, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Animals
Erythropoiesis / drug effects*
Humans
Injections, Subcutaneous
Iron / metabolism*
Macaca mulatta
Male
Recombinant Proteins / administration & dosage
Thrombocytopenia / metabolism,  physiopathology,  prevention & control*
Thrombopoietin / administration & dosage*
Whole-Body Irradiation
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 7439-89-6/Iron; 9014-42-0/Thrombopoietin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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