Document Detail


Prevention of hypothermic haloing extends the preservation time of hepatocytes at non freezing temperatures.
MedLine Citation:
PMID:  22884517     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Factors limiting the hypothermic preservation of hepatocytes on gelatin gels at 10°C were investigated. Following 4days of preservation, uniform morphological changes started to appear. The cells exhibited halos that increased in size. The particulate matter of the cell was confined to the central region. Cell viability was reduced from day 7 onwards. Neither fresh media changes nor the use of conditions to minimise free radical formation improved cell survival. However, haloing was decreased by short term temperature elevation to 37°C (3h), to reactivate the cells, and could be prevented completely by a stepwise increase in the sucrose concentration of the medium. The addition of sucrose in increments of 50mM, at four day intervals, was found to inhibit morphological change. Prevention of haloing enabled the cells to be preserved for at least two weeks. The preserved cells attached to supports and spread as if freshly isolated. The procedure allows extended preservation of cells at non-freezing temperatures.
Authors:
Peter J Evans
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-4
Journal Detail:
Title:  Cryobiology     Volume:  -     ISSN:  1090-2392     ISO Abbreviation:  Cryobiology     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Inc.
Affiliation:
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3AX, United Kingdom.
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