Document Detail

Prevention of hemolysis in rapidly frozen erythrocytes by using a laser pulse.
MedLine Citation:
PMID:  9917823     Owner:  NLM     Status:  MEDLINE    
This paper reports on the successful recovery of rapidly frozen unprotected erythrocytes by vitrification of the intracellular solution with a laser pulse prior to thawing. Erythrocytes that were frozen at 10,000 degrees C/min exhibited 100% hemolysis when thawed unless they were first irradiated by a 7 ns. laser pulse that selectively targeted the intracellular ice so that it was melted and resolidified into a glass phase. Up to 80% of the cells treated in this way remained intact after thawing. Wright's staining confirmed a healthy cell morphology and the retention of hemoglobin in the laser treated cells. While it is well known that small amounts of intracellular ice can be tolerated by cells, the findings of this study are the first to indicate that intracellular ice may be innocuous even when formed in substantial quantities provided that crystal growth and coalescence can somehow be avoided during warming.
A J Fowler; M Toner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  858     ISSN:  0077-8923     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1999-02-11     Completed Date:  1999-02-11     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  245-52     Citation Subset:  IM    
Mechanical Engineering Department, University of Massachusetts, Dartmouth 02747, USA.
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MeSH Terms
Blood Preservation / instrumentation,  methods*
Cryopreservation / instrumentation,  methods*
Erythrocytes / cytology,  physiology*,  radiation effects

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