| Prevention of haemarthrosis in a murine model of acute joint bleeding. | |
| | |
MedLine Citation:
|
PMID: 19149856 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Preservation of normal joint function in patients with haemophilia is a goal of modern therapy. Regular injections of anti-haemophilic factor concentrate reduce the risk of joint bleeding, the optimal regimen for which remains under investigation. The goals of the experiment described here are: (i) to assess the capacity of a murine model of severe haemophilic arthropathy to predict the likelihood of success of a test product to prevent joint bleeding and the complications that follow and (ii) to compare the effectiveness of recombinant human activated factor VII (rFVIIa) to recombinant human factor VIII (rFVIII) to prevent acute joint bleeding in the mouse model of haemarthrosis. Mice lacking expression of FVIII received a single intravenous injection of human rFVIII (280 U kg(-1)), rFVIIa (10 mg kg(-1)) or vehicle prior to blunt trauma injury to the knee joint. Mice receiving rFVIII and rFVIIa developed less injury-induced joint bleeding, swelling and loss of range of motion compared to mice pretreated with vehicle. Despite the reduction in clinical symptoms, synovial hyperplasia was evident in all groups after 7 days although less pronounced in mice receiving rFVIII and rFVIIa. The data under these experimental conditions demonstrate: (i) that this model can be used to evaluate novel therapies designed to prevent joint bleeding (prophylaxis) and (ii) both rFVIII and rFVIIa reduced acute haemarthrosis but did not completely prevent synovitis, the sequelae of blood induced joint injury. |
| | |
Authors:
|
L A Valentino; N Hakobyan; T Kazarian; B B Sorensen; M Tranholm |
Related Documents
:
|
23517706 - Team-level flexibility, work-home spillover, and health behavior. 23174856 - Effects of short-term dietary nitrate supplementation on blood pressure, o2 uptake kine... 22644406 - Rationale for cardiopulmonary exercise test in the assessment of surgical risk. 10378566 - Transbronchial biopsy in the presence of profound elevation of the international normal... 9302346 - Magnesium abolishes inadequate kinetics of frequency adaptation of the q-at interval in... 3751466 - Circulating growth hormone forms in type 1 diabetic subjects: comparison with normal su... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Haemophilia : the official journal of the World Federation of Hemophilia Volume: 15 ISSN: 1365-2516 ISO Abbreviation: Haemophilia Publication Date: 2009 Jan |
Date Detail:
|
Created Date: 2009-01-19 Completed Date: 2009-08-04 Revised Date: 2009-10-21 |
Medline Journal Info:
|
Nlm Unique ID: 9442916 Medline TA: Haemophilia Country: England |
Other Details:
|
Languages: eng Pagination: 314-9 Citation Subset: IM |
Affiliation:
|
The RUSH Hemophilia and Thrombophilia Center, Department of Pediatrics, Rush University Medical Center, Chicago, IL, USA. lvalentino@rush.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acute Disease Animals Disease Models, Animal* Drug Evaluation / methods Factor VIII / therapeutic use Factor VIIa / therapeutic use Hemarthrosis / etiology, pathology, prevention & control* Hyperplasia / pathology, prevention & control Knee Injuries / complications, pathology Mice Mice, Knockout Recombinant Proteins / therapeutic use Synovial Membrane / pathology Synovitis / pathology, prevention & control Wounds, Nonpenetrating / complications, pathology |
| Chemical | |
Reg. No./Substance:
|
0/Recombinant Proteins; 0/recombinant FVIIa; 9001-27-8/Factor VIII; EC 3.4.21.21/Factor VIIa |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Thrombin generation assay: a useful routine check-up tool in the management of patients with haemoph...
Next Document: Individual, social, environmental, and physical environmental correlates with physical activity amon...