| Prevention of focal intimal hyperplasia in rat vein grafts by using a tissue engineering approach. | |
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MedLine Citation:
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PMID: 9862280 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study focused on the role of blood flow in the formation of focal intimal hyperplasia in vein grafts, as well as the development of an engineering approach that can be used to eliminate disturbed blood flow and prevent blood flow-related focal intimal hyperplasia. A rat vein graft model was constructed by interposing a jugular vein into the abdominal aorta with end-to-end anastomoses. Locally disturbed flow was identified by analyzing particle streak-lines in methyl salicylate-cleared and perfused vein grafts in vitro with a physiological Reynolds number. At day 10, 20, and 30 after surgery, focal intimal hyperplasia of the vein grafts was examined using a histological approach and the density of alpha-actin positive cells was determined using immunohistological and fluorescent approaches. Results showed that apparent eddy blood flow formed at the proximal, but not at the distal, end of the vein grafts due to graft-host diameter mismatch and local geometric distortions, and was associated with apparent focal intimal hyperplasia. The thickness of the alpha-actin positive layers of the proximal vein grafts was significantly higher than that of the distal grafts (192 +/- 27 vs. 94 +/- 18 microm, 278 +/- 55 vs. 124 +/- 20 microm, and 288 +/- 24 vs. 131 +/- 23 microm for day 10, 20. and 30, respectively). The density of the alpha-actin positive cells, however, was similar between the proximal and the distal regions (3569 +/- 361 vs. 3285 +/- 343 cells/mm2, 5540 +/- 650 vs. 5376 + 887 cells/mm2, and 5465 +/- 791 vs. 5278 +/- 524 cells/mm2 for day 10, 20, and 30, respectively). When eddy blood flow was eliminated by matching the graft-host diameters using a tissue engineering approach, the average thickness of the alpha-actin positive layers of the proximal (71 +/- 15, 86 +/- 16, and 85 +/- 14 microm for day 10, 20, and 30, respectively) and the distal vein grafts (68 +/- 13, 80 +/- 14, and 79 +/- 13 microm for day 10, 20, and 30, respectively) was reduced significantly. The density of the alpha-actin positive cells was also reduced significantly in the proximal (2946 +/- 359, 3261 +/- 295, 3472 +/- 599 cells/mm2 for day 10, 20, and 30, respectively) and in the distal regions (3151 +/- 511, 3466 +/- 687, 3593 +/- 688 cells/mm2 for day 10, 20, and 30, respectively). The thickness of the alpha-actin positive layers and the density of the alpha-actin positive cells were not significantly different between the proximal and distal regions of the engineered vein grafts at each observation time. These results suggest that eddy flow may develop in vein grafts and may facilitate the formation of focal intimal hyperplasia, and the vascular tissue engineering approach developed in this study may be used to prevent blood flow-related focal intimal hyperplasia in vein grafts. |
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Authors:
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S Q Liu |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Atherosclerosis Volume: 140 ISSN: 0021-9150 ISO Abbreviation: Atherosclerosis Publication Date: 1998 Oct |
Date Detail:
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Created Date: 1999-03-04 Completed Date: 1999-03-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0242543 Medline TA: Atherosclerosis Country: IRELAND |
Other Details:
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Languages: eng Pagination: 365-77 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208-3107, USA. sliu@nwu.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Animals Aorta, Abdominal / surgery Arteriovenous Anastomosis Biomedical Engineering / methods* Blood Flow Velocity Cell Count Follow-Up Studies Graft Occlusion, Vascular / metabolism, pathology, physiopathology Hyperplasia / pathology, prevention & control Jugular Veins / metabolism, pathology, transplantation* Male Microscopy, Fluorescence Muscle, Smooth, Vascular / metabolism, pathology Random Allocation Rats Rats, Sprague-Dawley Tunica Intima / pathology* |
| Chemical | |
Reg. No./Substance:
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0/Actins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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