Document Detail

Prevention of cold ischemia/rewarming-induced ERK 1/2, p38 kinase and HO-1 activation by trophic factor supplementation of UW solution.
MedLine Citation:
PMID:  18538757     Owner:  NLM     Status:  MEDLINE    
We have previously shown that trophic factor supplementation (TFS) of University of Wisconsin (UW) solution reduced early apoptotic changes in vascular endothelial cells. Here, we examine the effect of TFS on cell signaling pathways related to cell growth, differentiation, and apoptosis after cold ischemic storage. In this study, the effect of TFS on the phosphorylation of signaling molecules ERK (extracellular regulated-signaling kinase) 1/2 and p38 MAPK (mitogen activated protein kinases) and of HO-1 (hemeoxygenase-1), relative to changes seen in unmodified UW solution, were determined by Western blot in cells stored under cold ischemic conditions. Primary cultures of canine kidney proximal tubule cells (CKPTC) and human umbilical vein endothelial cells (HUVEC) were used in this study. There was a significant decrease, relative to UW solution, after 1 min rewarming in ERK 1 and 2 activity in CKPTCs. For p38 MAPK, a significant decrease after 5 min rewarming was seen in CKPTC (p<0.05) while significant reductions relative to UW solution were seen in HUVECs after both 1 and 5 min rewarming (p<0.05). Phosphorylated HO-1 was also decreased by 43% and 50% in HUVECs, relative to UW solution, after 1 and 5 min rewarming (p<0.05 at each time point). Collectively, TFS not only limits ERK 1/2 and p38 MAPK activity induced by cold ischemic injury and subsequent rewarming, but also substantially restricted increases in HO-1 phosphorylation.
Young Sam Kwon; John D Foley; Paul Russell; Jonathan F McAnulty; Christopher J Murphy
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Publication Detail:
Type:  Journal Article     Date:  2008-04-25
Journal Detail:
Title:  Cryobiology     Volume:  57     ISSN:  1090-2392     ISO Abbreviation:  Cryobiology     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-01     Completed Date:  2008-10-31     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  72-4     Citation Subset:  IM    
Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, 2015 Linden Drive W, Madison, WI 53706-1102, USA.
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MeSH Terms
Adenosine / metabolism,  pharmacology
Allopurinol / metabolism,  pharmacology
Cells, Cultured
Cold Ischemia*
Extracellular Signal-Regulated MAP Kinases / metabolism*
Glutathione / metabolism,  pharmacology
Heme Oxygenase-1 / metabolism*
Insulin / metabolism,  pharmacology
Intercellular Signaling Peptides and Proteins / pharmacology
Kidney Tubules / cytology,  drug effects
Mitogen-Activated Protein Kinase 1 / metabolism
Mitogen-Activated Protein Kinase 3 / metabolism
Organ Preservation Solutions / metabolism,  pharmacology*
Raffinose / metabolism,  pharmacology
Signal Transduction
p38 Mitogen-Activated Protein Kinases / metabolism*
Reg. No./Substance:
0/Intercellular Signaling Peptides and Proteins; 0/Organ Preservation Solutions; 0/University of Wisconsin-lactobionate solution; 11061-68-0/Insulin; 315-30-0/Allopurinol; 512-69-6/Raffinose; 58-61-7/Adenosine; 70-18-8/Glutathione; EC Oxygenase-1; EC Signal-Regulated MAP Kinases; EC Protein Kinase 1; EC Protein Kinase 3; EC Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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